CD184

Rat IgG2a, κ

Human CXCR4 fusion protein

The monoclonal antibody 1D9 reacts with the human CD184 which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. CXCR4 was widely expressed by hematopoietic and non-hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The human CXCR4 gene has been mapped to chromosome 2q21. The immunogen used to generate 1D9 hybridoma was human CXCR4 fusion protein. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

1D9 The monoclonal antibody 1D9 reacts with the human CD184 which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. CXCR4 was widely expressed by hematopoietic and non-hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The human CXCR4 gene has been mapped to chromosome 2q21. The immunogen used to generate 1D9 hybridoma was human CXCR4 fusion protein. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Rat IgG2a, κ

克隆 1D9 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested)

Human (QC Testing)

CD184 (CXCR4)

CXCR4, Fusin

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(11) 1. Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology). 2. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology). 3. Federsppiel B, Melhado IG, Duncan AM, et al. Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated from human spleen. Genomics. 1993; 16(3):707-712. (Biology). 4. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology). 5. Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen). 6. Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology). 7. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997; 385(6617):645-649. (Biology). 8. Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology). 9. Loetscher M, Geiser T, O'Reilly T, Zwahlen R, Baggiolini M, Moser B. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J Biol Chem. 1994; 269(1):232-237. (Biology). 10. Nomura H, Nielsen BW, Matsushima K. Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors. Int Immunol. 1993; 5(10):1239-1249. (Biology). 11. Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology).