BD Pharmingen- Purified Mouse Anti-Rat CD63_BD Pharmingen
产品信息
抗原名称
CD63
宿主
Mouse BALB/c IgG1, κ
免疫原
Rat basophilic leukemia cell line
简单描述
The AD1 monoclonal antibody specifically recognizes a 50-60-kDa protein that is a homolog (87%) of human CD63 (melanomaassociated antigen ME491). CD63, a highly glycosylated type-III lysosomal glycoprotein, is a member of the tetraspan transmembrane 4 superfamily (TM4SF) that also includes CD9, CD37, and CD53. In the rat, CD63 is expressed on mast cells and platelets. CD63 is also found on rat embryonic fibroblasts and hepatic cell, along with cultured cell lines of diverse origin (eg, liver, lung, and heart). CD63 expression is upregulated with culture. CD63 is expressed in close proximity to the FcεRI on rat mast cells and has been shown to partially inhibit IgE-mediated histamine release. The function of CD63 has not been fully characterized.
商品描述
AD1
The AD1 monoclonal antibody specifically recognizes a 50-60-kDa protein that is a homolog (87%) of human CD63 (melanomaassociated antigen ME491). CD63, a highly glycosylated type-III lysosomal glycoprotein, is a member of the tetraspan transmembrane 4 superfamily (TM4SF) that also includes CD9, CD37, and CD53. In the rat, CD63 is expressed on mast cells and platelets. CD63 is also found on rat embryonic fibroblasts and hepatic cell, along with cultured cell lines of diverse origin (eg, liver, lung, and heart). CD63 expression is upregulated with culture. CD63 is expressed in close proximity to the FcεRI on rat mast cells and has been shown to partially inhibit IgE-mediated histamine release. The function of CD63 has not been fully characterized.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 AD1 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry, Immunoprecipitation (Reported)
反应种属
Rat (QC Testing)
目标/特异性
CD63
背景
别名
ME491; Cd63; mast cell antigen AD1
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
2. Boros M, Takaichi S, Masuda J, Newlands GF, Hatanaka K. Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat. Shock. 1995; 3(2):125-131. (Clone-specific: Immunohistochemistry).
3. Kitani S, Berenstein E, Mergenhagen S, Tempst P, Siraganian RP. A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491. J Biol Chem. 1991; 266(3):1903-1909. (Immunogen: Immunoprecipitation).
4. Nishikata H, Oliver C, Mergenhagen SE, Siraganian RP. The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture. J Immunol. 1992; 149(3):862-870. (Biology).
参考图片
The expression of CD63 on rat CD54+ peritoneal lavage cells. Lewis rat peritoneal lavage cells were incubated simultaneously with either purified Mouse IgG1, κ isotype control (Cat. no. 557273, open histogram) or purified AD1 mAb (shaded histogram), followed by biotinylated A85-1 mAb (rat anti-mouse IgG1, Cat. no. 553441) and finally Streptavidin-FITC (Cat. no. 554060) and PE-conjugated 1A29 mAb (anti-rat CD54, Cat. no. 554970). For analysis, a gate was drawn to include CD54+ cells only (gate not shown). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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