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BD Pharmingen- PE Mouse Anti-Human TNFR Related Protein -LT-R_BD Pharmingen

艾美捷科技 • 2025年08月20日 10:52:30 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
PE
抗原名称
TNFR Related Protein
宿主
Mouse IgG1, κ
免疫原
Human LTβR-Fc protein
简单描述
The hTNFR-RP-M12 monoclonal antibody specifically recognizes the extracellular domain of the 61 kDa receptor for the human cytokines, LTα1β2 and LIGHT. This receptor is referred as the Lymphotoxin β Receptor (LTβR). LTβR is encoded by TNFRSF3. LTβR was previously known as TNFRIII and TNF receptor-related protein (TNFRrp). LTβR is a type I transmembrane glycoprotein and member of the TNF Receptor Superfamily. LTβR are expressed on stromal cells in lymphoid tissue, normal dermal fibroblasts, bronchial airway epithelial cells and in a variety of adherent cell lines including FDC-1, U937, HT-29, HeLa and HEK 293 cells. LTβR are absent on human peripheral blood T and B cells and expressed at low levels by monocytes.
商品描述
hTNFR-RP-M12 The hTNFR-RP-M12 monoclonal antibody specifically recognizes the extracellular domain of the 61 kDa receptor for the human cytokines, LTα1β2 and LIGHT. This receptor is referred as the Lymphotoxin β Receptor (LTβR). LTβR is encoded by TNFRSF3. LTβR was previously known as TNFRIII and TNF receptor-related protein (TNFRrp). LTβR is a type I transmembrane glycoprotein and member of the TNF Receptor Superfamily. LTβR are expressed on stromal cells in lymphoid tissue, normal dermal fibroblasts, bronchial airway epithelial cells and in a variety of adherent cell lines including FDC-1, U937, HT-29, HeLa and HEK 293 cells. LTβR are absent on human peripheral blood T and B cells and expressed at low levels by monocytes.
同种型
Mouse IgG1, κ
克隆号
克隆 hTNFR-RP-M12 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
TNFR Related Protein
背景
别名
LT-BETA-R; LTBR; Lymphotoxin-beta receptor; TNF-RIII; TNFRSF3; TNFR-RP
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Browning JL, Dougas I, Ngam-ek A, et al. Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors.. J Immunol. 1995; 154(1):33-46. (Immunogen). 2. Crowe PD, VanArsdale TL, Walter BN, et al. A lymphotoxin-beta-specific receptor. Science. 1994; 264(5159):707-710. (Biology). 3. Murphy M, Walter BN, Pike-Nobile L, . Expression of the lymphotoxin beta receptor on follicular stromal cells in human lymphoid tissues. Cell Death Differ. 1998; 5(6):497-505. (Biology). 4. Rooney IA, Butrovich KD, Glass AA, et al. The lymphotoxin-beta receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells. J Biol Chem. 2000; 275(19):14307-14315. (Biology). 5. Tamada K, Shimozaki K, Chapoval AI, . LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response. J Immunol. 2000; 164(8):4105-4110. (Biology). 6. Zhai Y, Guo R, Hsu TL, et al. LIGHT, a novel ligand for lymphotoxin beta receptor and TR2/HVEM induces apoptosis and suppresses in vivo tumor formation via gene transfer.. J Clin Invest. 1998; 102(16):1142-1151. (Biology).

参考图片

Flow cytometric analysis of LTβR expression by HeLa, Jurkat and human monocytes. HeLa cell line (Left Panel), Jurkat cell line (Middle Panel), or human peripheral monocytes (Right Panel) were stained with PE Mouse Anti-Human TNFR Related Protein (LTβR) (Cat No. 551503; filled histograms) or PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; empty histograms) at 0.5 µg/10^6 cells. Whole human blood was first treated with PharmLyse™ Lysing Buffer (Cat No. 555899) to lyse erythrocytes and blocked with normal polyclonal human IgG (5 µg/10^6 cells) prior to staining. Note that certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

Flow cytometric analysis of LTβR expression by HeLa, Jurkat and human monocytes. HeLa cell line (Left Panel), Jurkat cell line (Middle Panel), or human peripheral monocytes (Right Panel) were stained with PE Mouse Anti-Human TNFR Related Protein (LTβR) (Cat No. 551503; filled histograms) or PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; empty histograms) at 0.5 µg/10^6 cells. Whole human blood was first treated with PharmLyse™ Lysing Buffer (Cat No. 555899) to lyse erythrocytes and blocked with  normal polyclonal human IgG (5 µg/10^6 cells) prior to staining. Note that certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

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