BD Pharmingen- APC Mouse Anti-Human CD209_BD Pharmingen
产品信息
荧光素标记
APC
抗原名称
CD209
宿主
Mouse IgG2b, κ
免疫原
Human Monocyte Derived DC Cells
简单描述
The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain. It is highly expressed on dendritic cells in mucosal tissues. Its sequence is identical to the HIV-1 envelope gp120-binding C-type lectin, and reports suggest that DC-SIGN binds to HIV-1 gp120 and effectively transmits infectious HIV-1 to resting T lymphocytes expressing CD4 and chemokine receptors. The C-type lectin domain of DC-SIGN is also capable of binding other pathogenic viruses, bacteria, and parasites. Reports also suggest that DC-SIGN enables the highly efficient migration of dendritic cells from blood into the tissues. It can interact with ICAM-2, which has a similar sequence as ICAM-3, and is abundantly expressed on vascular and lymphoid endothelium. Thus, DC-SIGN mediates dendritic cells rolling and transendothelial migration, and its interaction with ICAM-2 is essential to specific migratory functions of dendritic cells.
商品描述
DCN46
The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain. It is highly expressed on dendritic cells in mucosal tissues. Its sequence is identical to the HIV-1 envelope gp120-binding C-type lectin, and reports suggest that DC-SIGN binds to HIV-1 gp120 and effectively transmits infectious HIV-1 to resting T lymphocytes expressing CD4 and chemokine receptors. The C-type lectin domain of DC-SIGN is also capable of binding other pathogenic viruses, bacteria, and parasites. Reports also suggest that DC-SIGN enables the highly efficient migration of dendritic cells from blood into the tissues. It can interact with ICAM-2, which has a similar sequence as ICAM-3, and is abundantly expressed on vascular and lymphoid endothelium. Thus, DC-SIGN mediates dendritic cells rolling and transendothelial migration, and its interaction with ICAM-2 is essential to specific migratory functions of dendritic cells.
同种型
Mouse IgG2b, κ
克隆号
克隆 DCN46 (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD209 (DC-SIGN)
背景
别名
DC-SIGN
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Appelmelk BJ, van Die I, van Vliet SJ, et al. Carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells. J Immunol. 2003; 170(4):1635-1639. (Biology).
2. Baribaud F, Pohlmann S, Sparwasser T, et al. Functional and antigenic characterization of human, rhesus macaque pigtailed macaque, and murine DC-SIGN. J Virol. 2001; 75(21):10281-10289. (Biology).
3. Gruber A, Chalmers AS, Popov S, Ruprecht RM. Functional aspects of binding of monoclonal antibody DCN46 to DC-SIGN on dendritic cells.. Immunol Lett. 2002; 84(2):103-8. (Clone-specific).
4. Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995; 182(2):389-400. (Immunogen).
5. Steinman RM, Granelli-Piperno A, Pope M, et al. The interaction of immunodeficiency viruses with dendritic cells. Curr Top Microbiol Immunol. 2003; 276:1-30. (Biology).
6. Steinman RM. DC-SIGN: a guide to some mysteries to dendritic cells. Cell. 2000; 100(5):491-494. (Biology).
参考图片
Flow cytometric analysis of CD209 expression on peripheral blood monocyte-derived dendritic cells. Adherent peripheral blood mononuclear cells were cultured for 7 days with the recombinant human cytokines, GM-CSF (Cat. No. 550068), TNF (Cat. No. 554618), and IL-4 (Cat. No. 554605). The cultured dendritic cells were harvested and stained with either APC Mouse IgG2 b, κ Isotype Control (Cat. No. 555745, open histogram) or APC Mouse Anti-Human CD209 (Cat. No. 551545; filled histogram).
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/8985.html