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BD Pharmingen- Purified Rat Anti-Pig - T Lymphocytes_BD Pharmingen

艾美捷科技 • 2025年08月20日 11:01:11 • 抗体 • 阅读 8 • 标签
产品信息
抗原名称
γδ T Cell
宿主
Rat PVG, also known as HO, Black Hooded IgG2a, κ
免疫原
Null T cells purified from the blood of young pigs
简单描述
The MAC320 antibody monoclonal antibody specifically binds to the 270- and 280-kDa isoforms of the SWC6 heterodimer expressed on Null T cells. These cells form a unique population of CD2-, surface Ig-, and thymus-dependent nylon wool-nonadherent peripheral blood lymphocytes found at high frequencies in young pigs. Null T cells are also CD4- and CD8-, and the majority of them express γδ T-cell receptors (TCR). In addition to peripheral blood, MAC320+ leukocytes (CD2- γδ T cells) are found in the thymus, especially in the medulla and in the marginal zone and red pulp of the spleen. They are rare in normal lymph nodes, tonsils, Peyer's patches, bone marrow, and skin. An additional population of MAC320- CD2+ γδ T cells is found in the blood and spleen. The MAC320 antibody does not react with pig erythrocytes, monocytes, or granulocytes, nor with cow, sheep, or human lymphocytes.
商品描述
MAC320 The MAC320 antibody monoclonal antibody specifically binds to the 270- and 280-kDa isoforms of the SWC6 heterodimer expressed on Null T cells. These cells form a unique population of CD2-, surface Ig-, and thymus-dependent nylon wool-nonadherent peripheral blood lymphocytes found at high frequencies in young pigs. Null T cells are also CD4- and CD8-, and the majority of them express γδ T-cell receptors (TCR). In addition to peripheral blood, MAC320+ leukocytes (CD2- γδ T cells) are found in the thymus, especially in the medulla and in the marginal zone and red pulp of the spleen. They are rare in normal lymph nodes, tonsils, Peyer's patches, bone marrow, and skin. An additional population of MAC320- CD2+ γδ T cells is found in the blood and spleen. The MAC320 antibody does not react with pig erythrocytes, monocytes, or granulocytes, nor with cow, sheep, or human lymphocytes.
同种型
Rat PVG, also known as HO, Black Hooded IgG2a, κ
克隆号
克隆 MAC320 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Reported), Cytotoxicity (Not Recommended)
反应种属
Pig (QC Testing)
目标/特异性
γδ T Cell
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5) 1. Binns RM, Duncan IA, Powis SJ, Hutchings A, Butcher GW. Subsets of null and gamma delta T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies. Immunology. 1992 October; 77(2):219-227. (Immunogen: Cytotoxicity, Immunoprecipitation). 2. Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry). 3. Binns RM. The Null/gamma delta TCR+ T cell family in the pig. Vet Immunol Immunopathol. 1994 October; 43(1-3):69-77. (Biology). 4. Davis WC, Zuckermann FA, Hamilton MJ, et al. Analysis of monoclonal antibodies that recognize gamma delta T/null cells. Vet Immunol Immunopathol. 1998 January; 60(3-4):305-316. (Biology). 5. Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology).

参考图片

Two-color analysis of pig peripheral blood γδ T lymphocytes. Pig whole blood was stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no.553927, left panel) or purified mAb MAC320 (center and right anels), followed by biotinylated mouse anti-rat IgG2a mAb RG7/1.30 (Cat. no. 553894, all panels) then Streptavidin-PE (Cat. no.554061, all panels). In the right panel, anti-pig γδ TCR mAb PGBL22A (VMRD, Pullman, WA) was added in a fourth staining step, followed by FITC-conjugated rat anti-mouse IgG1 mAb A85-1. Erythrocytes were lysed (PharM Lyse™, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815), and lymphocytes were gated according to their ligh-scatter profile. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA). The right panel demonstrates that MAC320+ cells express the gd TCR and that there are MAC320+ and MAC320- subpopulations of γδ TCR-bearing T lymphocytes in the blood.

Two-color analysis of pig peripheral blood γδ T lymphocytes. Pig whole blood was stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no.553927, left panel) or purified mAb MAC320 (center and right anels), followed by biotinylated mouse anti-rat IgG2a mAb RG7/1.30 (Cat. no. 553894, all panels) then Streptavidin-PE (Cat. no.554061, all panels). In the right panel, anti-pig γδ TCR mAb PGBL22A (VMRD, Pullman, WA) was added in a fourth staining step, followed by FITC-conjugated rat anti-mouse IgG1 mAb A85-1. Erythrocytes were lysed (PharM Lyse™, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815), and lymphocytes were gated according to their ligh-scatter profile. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA). The right panel demonstrates that MAC320+ cells express the gd TCR and that there are MAC320+ and MAC320- subpopulations of γδ TCR-bearing T lymphocytes in the blood.

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