IFN-α

Mouse IgG1, κ

Human IFN-α2b

The 7N4-1 antibody reacts with human IFN-α2b and to a lesser extent with IFN-α7.  It does not react with IFN-α1 nor IFN-α4.  IFN-α2b is one of the three variants of IFN-α2 that have been isolated from human cell lines. IFN-α2b is the variant predominantly produced by human leukocytes.  Human IFN-α2b belongs to the IFN-α class of proteins also known as leukocyte interferons. IFN-α comprises a family of related but distinct proteins with molecular weights ranging from 16-27 kDa with antiviral, antiproliferative and immunomodulatory activities.  The IFN-α family is composed from as many as 14 different genes.  The immunogen used to generate the 7N4-1 hybridoma was E. coli-expressed recombinant human IFN-α2b. This is a neutralizing antibody.

7N4-1 The 7N4-1 antibody reacts with human IFN-α2b and to a lesser extent with IFN-α7.  It does not react with IFN-α1 nor IFN-α4.  IFN-α2b is one of the three variants of IFN-α2 that have been isolated from human cell lines. IFN-α2b is the variant predominantly produced by human leukocytes.  Human IFN-α2b belongs to the IFN-α class of proteins also known as leukocyte interferons. IFN-α comprises a family of related but distinct proteins with molecular weights ranging from 16-27 kDa with antiviral, antiproliferative and immunomodulatory activities.  The IFN-α family is composed from as many as 14 different genes.  The immunogen used to generate the 7N4-1 hybridoma was E. coli-expressed recombinant human IFN-α2b. This is a neutralizing antibody.

Mouse IgG1, κ

克隆 7N4-1 (RUO)

1.0 mg/ml

Intracellular staining (flow cytometry) (Routinely Tested), Neutralization (Tested During Development), (Reported)

Human (QC Testing)

IFN-α

IFNa, IFNα

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

研发参考(8) 1. Dipaola M, Smith T, Ferencz-Biro K, Liao MJ, Testa D.. Interferon-alpha 2 produced by normal human leukocytes is predominantly interferon-alpha 2b. J Interferon Res. 1994; 14(6):325-332. (Biology). 2. Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Methodology: Neutralization). 3. Lydon NB, Favre C, Bove S, et al. Immunochemical mapping of alpha-2 interferon. Biochemistry. 1985; 24(15):4131-4141. (Immunogen). 4. Pestka S. Interferon from 1981 to 1986. Methods Enzymol. 1986; 119:3-14. (Biology). 5. Pestka S. The human interferon alpha species and receptors. 2000; 55(4):254-287. (Biology). 6. Pestka S. The human interferons—from protein purification and sequence to cloning and expression in bacteria: before, between, and beyond. Arch Biochem Biophys. 1983; 221(1):1-37. (Biology). 7. Siegal FP, Kadowaki N, Shodell M, et al. The nature of the principal type 1 interferon-producing cells in human blood. Science. 1999; 284(5421):1835-1837. (Clone-specific: Immunocytochemistry (cytospins)). 8. Vogel S, Friedman R, Hogan M. Measurement of antiviral activity induced by interferons a, b, and g.. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley & Sons; 2007:6.9.1-6.9.15.