BD Pharmingen- PE Rat IgG2a- - Isotype Control_BD Pharmingen
产品信息
荧光素标记
PE
免疫原
Mouse Pooled Immunoglobulin
简单描述
The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.
This reagent has been used as an isotype control in immunophenotypic studies with baboon, rhesus or cynomolgus peripheral blood cells.
商品描述
R35-95
The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.
This reagent has been used as an isotype control in immunophenotypic studies with baboon, rhesus or cynomolgus peripheral blood cells.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
克隆号
克隆 R35-95 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
推荐用量
20 µl
目标/特异性
Rat IgG2a κ Isotype Ctrl
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
Profile of PMA-stimulated (24 hours) permeabilized peripheral blood lymphocytes of Rhesus macaque monkey (macaca mulatta) analyzed by flow cytometry
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