产品信息
荧光素标记
FITC
抗原名称
CD42d
宿主
Mouse BALB/c IgG2a, κ
免疫原
Wistar rat platelets
简单描述
The RPM.4 monoclonal antibody specifically recognizes CD42d (Platelet glycoprotein V, GPV). CD42d is a 88-kDa type I transmembrane glycoprotein which noncovalently associates with GPIb and GPIX to form a receptor for von Willebrand factor on megakaryocytes and resting platelets. The binding sites for von Willebrand factor and thrombin have been localized to the GPIbα chain of the GPI-b-V-IX complex. GPV is cleaved by thrombin to release a 70 kDa soluble fragment, which contains the epitope recognized by mAb RPM.4. Although the rat CD42d protein's structure is similar to that of human CD42d, the RPM.4 antibody does not cross-react with human platelets.
商品描述
The RPM.4 monoclonal antibody specifically recognizes CD42d (Platelet glycoprotein V, GPV). CD42d is a 88-kDa type I transmembrane glycoprotein which noncovalently associates with GPIb and GPIX to form a receptor for von Willebrand factor on megakaryocytes and resting platelets. The binding sites for von Willebrand factor and thrombin have been localized to the GPIbα chain of the GPI-b-V-IX complex. GPV is cleaved by thrombin to release a 70 kDa soluble fragment, which contains the epitope recognized by mAb RPM.4. Although the rat CD42d protein"s structure is similar to that of human CD42d, the RPM.4 antibody does not cross-react with human platelets.
同种型
Mouse BALB/c IgG2a, κ
克隆号
RPM.4
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Rat (QC Testing)
背景
别名
PLGPV; Gp5; platelet glycoprotein V; GPV; Platelet glycoprotein 5
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Ravanat C, Morales M, Azorsa DO, et al. Gene cloning of rat and mouse platelet glycoprotein V: identification of megakaryocyte-specific promoters and demonstration of functional thrombin cleavage.. Blood. 1997; 89(9):3253-62. (Immunogen).
参考图片
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