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BD Pharmingen- Purifed Rat Anti-Human CXCR5 -CD185_BD Pharmingen

艾美捷科技 • 2025年08月20日 11:47:13 • 抗体 • 阅读 55 • 标签
产品信息
抗原名称
CD185 (CXCR5)
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
免疫原
Human CXCR5 (CD185)
简单描述
The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5.
商品描述
RF8B2 The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
克隆号
克隆 RF8B2 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD185 (CXCR5)
背景
别名
CD185; Chemokine C-X-C motif receptor 5; BLR1; MDR-15
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B. Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J. 1995; 309(3):773-779. (Biology). 2. Dobner T, Wolf I, Emrich T, Lipp M. Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma. Eur J Immunol. 1992; 22(11):2795-2799. (Biology). 3. Forster R, Emrich T, Kremmer E, Lipp M. Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells. Blood. 1994; 84(3):830-840. (Immunogen). 4. Gunn MD, Ngo VN, Ansel KM, Ekland EH, Cyster JG, Williams LT. A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature. 1998; 391(6669):799-803. (Biology). 5. Kouba M, Vanetti M, Wang X, Schafer M, Hollt V. Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue. FEBS Lett. 1993; 321(2-3):173-178. (Biology). 6. Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B. B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med. 1998; 187(4):655-660. (Biology).
数据库链接
Entrez-Gene ID
643

参考图片

Detection of CXCR5 (CD185) expression on human peripheral lymphocytes by Purified Rat-Anti Human CXCR5 (CD185). Human peripheral blood mononuclear cells were stained with 0.25 µg/test of Purified Rat Anti-Human CXCR5 (CD185) (Cat. No. 552032), using the 3-step staining protocol outlined below, and FITC Mouse Anti-Human CD3 (Cat. No. 555339; left panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412; right panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The data reflects gating on lymphocytes based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Purified Rat IgG2b, ĸ Isotype Control (Cat. No. 555846) and FITC Mouse IgG2a ĸ Isotype Control (Cat. No. 555573), or FITC Mouse IgG1, κ Isotype (Cat. No. 555748). The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed using a BD FACScan™ Flow Cytometer (BD Biosciences, San Jose, CA)

Detection of CXCR5 (CD185) expression on human peripheral lymphocytes by Purified Rat-Anti Human CXCR5 (CD185). Human peripheral blood mononuclear cells were stained with 0.25 µg/test of Purified Rat Anti-Human CXCR5 (CD185) (Cat. No. 552032), using the 3-step staining protocol outlined below, and FITC Mouse Anti-Human CD3 (Cat. No. 555339; left panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412; right panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The data reflects gating on lymphocytes based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Purified Rat IgG2b, ĸ Isotype Control (Cat. No. 555846) and FITC Mouse IgG2a ĸ Isotype Control (Cat. No. 555573), or FITC Mouse IgG1, κ Isotype (Cat. No. 555748). The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed using a BD FACScan™ Flow Cytometer (BD Biosciences, San Jose, CA)

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