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BD Pharmingen- PE Rat Anti-Mouse CD278_BD Pharmingen

艾美捷科技 • 2025年08月20日 13:07:43 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
PE
抗原名称
CD278
宿主
Rat IgG2b, κ
免疫原
Mouse Icos cDNA and ICOS hexahistidine fusion protein
简单描述
The 7E.17G9 monoclonal antibody specifically binds to CD278, the I nducible Cos timulatory molecule (ICOS), a 47-57 kDa homodimeric glycoprotein of the CD28 family of costimulatory molecules. ICOS is expressed on subpopulations of CD4-CD8- and CD4+CD8- (but not CD4-CD8+ or CD4+CD8+) thymocytes, on some T-cell lines, and on small numbers of peripheral leukocytes. It is upregulated on T lymphocytes following activation via the T-cell receptor. The T-cell activation marker H4 is the same molecule as  ICOS. ICOS is a costimulatory receptor, and its ligand on antigen-presenting cells has been called B7RP-1, GL50, B7h, B7-H2, or LICOS. There is considerable evidence that the interaction of ICOS with its ligand is involved in the regulation of many, but not all, T-cell-mediated immune responses.
商品描述
7E.17G9 The 7E.17G9 monoclonal antibody specifically binds to CD278, the I nducible Cos timulatory molecule (ICOS), a 47-57 kDa homodimeric glycoprotein of the CD28 family of costimulatory molecules. ICOS is expressed on subpopulations of CD4-CD8- and CD4+CD8- (but not CD4-CD8+ or CD4+CD8+) thymocytes, on some T-cell lines, and on small numbers of peripheral leukocytes. It is upregulated on T lymphocytes following activation via the T-cell receptor. The T-cell activation marker H4 is the same molecule as  ICOS. ICOS is a costimulatory receptor, and its ligand on antigen-presenting cells has been called B7RP-1, GL50, B7h, B7-H2, or LICOS. There is considerable evidence that the interaction of ICOS with its ligand is involved in the regulation of many, but not all, T-cell-mediated immune responses.
同种型
Rat IgG2b, κ
克隆号
克隆 7E.17G9 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD278 (ICOS)
背景
别名
ICOS; Ailim; CCLP; CRP-1; Ly115; Inducible T-cell costimulator
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(9) 1. Buonfiglio D, Bragardo M, Redoglia V, et al. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 2000; 30(12):3463-3467. (Biology). 2. Chambers CA. The expanding world of co-stimulation: the two-signal model revisited. Trends Immunol. 2001; 22(4):217-223. (Biology). 3. Dong C, Temann UA, Flavell RA. Cutting edge: critical role of inducible costimulator in germinal center reactions. J Immunol. 2001; 166(6):3659-3662. (Biology). 4. Gonzalo JA, Delaney T, Corcoran J, Goodearl A, Gutierrez-Ramos JC, Coyle AJ. Cutting edge: the related molecules CD28 and inducible costimulator deliver both unique and complementary signals required for optimal T cell activation. J Immunol. 2001; 166(1):1-5. (Biology). 5. Mages HW, Hutloff A, Heuck C, et al. Molecular cloning and characterization of murine ICOS and identification of B7h as ICOS ligand. Eur J Immunol. 2000; 30(4):1040-1047. (Biology). 6. McAdam AJ, Chang TT, Lumelsky AE, et al. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells.. J Immunol. 2000; 165(9):5035-40. (Immunogen). 7. Schwartz RH. Immunology. It takes more than two to tango. Nature. 2001; 409(6816):31-32. (Biology). 8. Sperling AI, Bluestone JA. ICOS costimulation: It's not just for TH2 cells anymore. Nat Immunol. 2001; 2(7):573-574. (Biology). 9. Wallin JJ, Liang L, Bakardjiev A, Sha WC. Enhancement of CD8+ T cell responses by ICOS/B7h costimulation. J Immunol. 2001; 167(1):132-139. (Biology).

参考图片

The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated (top panels) or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb (anti-mouse CD3e, Cat. No. 553057, bottom panels). Both sets of cells were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panels) or PE-conjugated mAb 7E.17G9 (right panels), then FITC-conjugated mAb 53-6.7 (anti-mouse CD8a, Cat. No. 553030/553031). Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA).

The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated (top panels) or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb (anti-mouse CD3e, Cat. No. 553057, bottom panels). Both sets of cells were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panels) or PE-conjugated mAb 7E.17G9 (right panels), then FITC-conjugated mAb 53-6.7 (anti-mouse CD8a, Cat. No. 553030/553031). Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA).

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