1. 首页
  2. 抗体
  3. 正文

BD Pharmingen- Purified Mouse Anti-Mouse Pre-T Cell Receptor - Chain_BD Pharmingen

艾美捷科技 • 2025年08月20日 13:23:53 • 抗体 • 阅读 7 • 标签
产品信息
抗原名称
Pre-TCR α Chain
宿主
Mouse C57BL/6 IgG1, κ
免疫原
Recombinant extracellular (Ig-like) domain of pTα
简单描述
At very early stages in the intrathymic differentiation of T lymphocytes which will bear αβ T Cell Receptors (TCR), the TCR β chain forms a heterodimer with the pre-TCR α chain (pTα) to form the pre-TCR. This pre-TCR associates with the signal-transducing CD3 complex and controls the survival and proliferation of CD4- CD8- (DN) thymocytes, plays a role in allelic exclusion of the TCR β chain, directly or indirectly regulates TCR δ chain expression, and is eventually replaced by the TCR α chain as thymocytes mature. The 2F5 monoclonal antibody specifically recognizes the PTα on the surface of CD44- CD25-/lo DN thymocytes.
商品描述
2F5 At very early stages in the intrathymic differentiation of T lymphocytes which will bear αβ T Cell Receptors (TCR), the TCR β chain forms a heterodimer with the pre-TCR α chain (pTα) to form the pre-TCR. This pre-TCR associates with the signal-transducing CD3 complex and controls the survival and proliferation of CD4- CD8- (DN) thymocytes, plays a role in allelic exclusion of the TCR β chain, directly or indirectly regulates TCR δ chain expression, and is eventually replaced by the TCR α chain as thymocytes mature. The 2F5 monoclonal antibody specifically recognizes the PTα on the surface of CD44- CD25-/lo DN thymocytes.
同种型
Mouse C57BL/6 IgG1, κ
克隆号
克隆 2F5 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
Pre-TCR α Chain
背景
别名
Ptcra; pTα; pTa; gp33; pT[a]; pTalpha; pT-alpha; pT-alpha-TCR
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(7) 1. Aifantis I, Azogui O, Feinberg J, Saint-Ruf C, Buer J, von Boehmer H. On the role of the pre-T cell receptor in alphabeta versus gammadelta T lineage commitment. Immunity. 1998; 9(5):649-688. (Immunogen: Immunoprecipitation). 2. Aifantis I, Feinberg J, Fehling HJ, Di Santo JP, von Boehmer H. Early T cell receptor beta gene expression is regulated by the pre-T cell receptor-CD3 complex. J Exp Med. 1999; 190(1):141-144. (Biology). 3. Aifantis I, Pivniouk VI, Gartner F, et al. Allelic exclusion of the T cell receptor beta locus requires the SH2 domain-containing leukocyte protein (SLP)-76 adaptor protein. J Exp Med. 1999; 190(8):1093-1102. (Biology). 4. Mancini S, Candeias SM, Fehling HJ, von Boehmer H, Jouvin-Marche E, Marche PN. TCR alpha-chain repertoire in pTalpha-deficient mice is diverse and developmentally regulated: implications for pre-TCR functions and TCRA gene rearrangement. J Immunol. 1999; 163(11):6053-6059. (Biology). 5. Wiest DL, Berger MA, Carleton M. Control of early thymocyte development by the pre-T cell receptor complex: A receptor without a ligand. Semin Immunol. 1999; 11(4):251-262. (Biology). 6. von Boehmer H, Aifantis I, Feinberg J, et al. Pleiotropic changes controlled by the pre-T-cell receptor. Curr Opin Immunol. 1999; 11(2):135-142. (Biology). 7. von Boehmer H, Fehling HJ. Structure and function of the pre-T cell receptor. Annu Rev Immunol. 1997; 15:433-452. (Biology).

参考图片

Multicolor analysis of the expression of pTα on thymocyte subsets. C57BL/6 thymocytes were stained with either purified mAb 2F5 (top right panel, bottom panels, (C-F) open histograms) or purified mouse IgG1, κ isotype control MOPC-31C (Cat. No. 557273, filled histograms) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb, Cat. No. 553141/533142), followed by biotinylated anti-mouse IgG1 mAb A85-1 (Cat. No. 553441), then Streptavidin-PE (Cat. No. 554061). FITC-conjugated anti-mouse CD44 mAb IM7 (Cat. No. 553133) and APC-conjugated anti-mouse CD25 mAb PC61 (Cat. No. 557192) were added in a fourth step. PerCP-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553052) and anti-mouse CD8a mAb 53-6.7 (Cat. No. 553036) were included in the fifth staining step. For data analysis, CD4- CD8a- (DN) thymocytes were gated as indicated in the top left panel. The expression pattern of CD44 and CD25 in that DN population is displayed in the top middle panel. The remaining panels display histograms of pTα expression on the gated subpopulations of DN thymocytes:  Panel C, CD44-/lo CD25hi;  Panel D, CD44- CD25lo; Panel E, CD44- CD25-; and Panel F, CD44+ CD25-.  Flow cytometry was performed on a BD FACSCalibur™ System (BD Biosciences, San Jose, CA).

文档下载: W 导出为BD Pharmingen- Purified Mouse Anti-Mouse Pre-T Cell Receptor - Chain_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9056.html

赞 (7)
打赏 微信扫一扫
« 上一篇 2025年08月20日 13:23:17
下一篇 » 2025年08月20日 13:24:33

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();