BD Pharmingen- Purified Mouse Anti-6XHIS with Control_BD Pharmingen
产品信息
抗原名称
6X Histone
简单描述
The engineering of a short epitope tag to a recombinant protein provides a very convenient method for detecting and isolating the protein of interest. For example, the placement of a series of 6 consecutive histidine residues that are incorporated at either end of the recombinant fusion protein facilitates the purification of that protein. Purification can be easily accomplished by metal chelate affinity chromatography using a metal resin column. The 6xHis monoclonal antibody can be used to detect the epitope-tagged protein of interest.
克隆号
克隆 F24-796 (RUO)
BD化合物表
- 描述数量/尺寸零件号EntrezGene ID
- 6xHis Antibody50 µg (1 ea)51-9000012N/A
- 6xHis Control Lysate100 µL (1 ea)51-9000129N/A
应用
实验应用
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
目标/特异性
6X Histone
文献
文献
研发参考(1)
1. Hochuli E, Dobeli H, Schacher A. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr. 1987; 411:177-184. (Biology).
参考图片
Western blot analysis of 6xHis expression on recombinant E. Coli-produced lysate. An E coli-produced recombinant 6xHis control lysate was probed with Purified Mouse Anti-6XHIS antibody (Cat. No. 552565) at the following concentrations: 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). 6xHis expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002). 6xHis control is identified as a band of ~31 kDa.
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9073.html