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BD Pharmingen- Purified Mouse Anti-NHP CD45_BD Pharmingen

艾美捷科技 • 2025年08月20日 13:42:39 • 抗体 • 阅读 6 • 标签
产品信息
抗原名称
CD45
宿主
Mouse IgG1, κ
免疫原
Rhesus peripheral whole blood
简单描述
D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.
商品描述
D058-1283 D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.
同种型
Mouse IgG1, κ
克隆号
克隆 D058-1283 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Rhesus, Cynomolgus, Baboon (QC Testing)
目标/特异性
CD45
背景
别名
Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Kishihara K, Penninger J, Wallace VA, et al. Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell. 1993; 74(1):143-156. (Biology). 2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 3. Reimann KA, Waite BC, Lee-Parritz DE, et al. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994; 17(1):102-108. (Biology).

参考图片

Western Blot analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 556648; lanes 1 and 4), Purified Mouse Anti-NHP CD45 (clone D058-1283; Cat. No. 552566; lanes 2 and 5), or Purified Mouse Anti-Human CD45 (clone TÜ116; lanes 3 and 6). Samples either non-reduced and shown at 1 second exposure (lanes 1-3), or reduced and shown at 10 seconds exposure (lanes 4-6). Staining was visualized with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).

Profile of anti-NHP CD45 (D058-1283) reactivity on peripheral blood of Rhesus macaque (Macaca mulatta) monkey analyzed by flow cytometry. Second step staining with Cat. No. 555988.

Western Blot analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 556648; lanes 1 and 4), Purified Mouse Anti-NHP CD45 (clone D058-1283; Cat. No. 552566; lanes 2 and 5), or Purified Mouse Anti-Human CD45 (clone TÜ116; lanes 3 and 6). Samples either non-reduced and shown at 1 second exposure (lanes 1-3), or reduced and shown at 10 seconds exposure (lanes 4-6). Staining was visualized with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).

Flow cytometric analysis of CD45 expression on peripheral blood of Rhesus macaque (Macaca mulatta). Rhesus blood was stained with Purified Mouse Anti-NHP CD45 (clone D058-1283), followed by FITC Goat Anti-Mouse IgG/IgM. The dot plot depicting side scatter versus CD45 expression was derived from gated events with the side and forward light-scattering characteristics of viable cells.

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