BD Pharmingen- Purified Hamster Anti-Mouse IL-12 Receptor -2_BD Pharmingen
参考图片
Expression of cell surface IL-12R 2 by T helper cells. Mouse Th1 cell line, 2D6 (left panel) and Th2 cell line, D10 (center panel) were stained with purified anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, 0.5 µg/test) followed by PE-conjugated anti-hamster IgG (0.25 µg, Cat . No. 554056). Staining with the HAM10B9 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The histograms in the figure were derived from gated events with the forward and side light scatter characteristics of viable lymphocytes. Mouse splenocytes from C57BL/6 mice (right panel) were treated with an ammonium chloride lysing buffer to remove the red blood cells. Cells were subsequently cultured with ConA (2 µg/ml), PMA (50 ng/ml), Dextran sulfate (10 µg/ml), LPS (5 µg/ml), recombinant mouse IL-2 (10 ng/ml), recombinant mouse IL-12p70 (20 ng/ml) and anti-IL-4 antibody, clone 11B11 (5 µg/ml) for 5 days. Following culture the cells were harvested, washed, blocked with mouse Fc Block™ (Cat. No. 553141) and stained with purified anti-mouse IL-12 receptor β2 antibody (clone HAM10B9, 0.5 µg/test) followed by PE-conjugated anti-hamster IgG (0.25 µg, Cat . No. 554056) and Viaprobe (Cat. No. 555816). Staining with anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, filled histograms) is compared to staining obtained using the isotype control antibody (Cat. No. 553969, open histograms). The histograms in the figure were derived from viable gated events (e.g. ViaProbe negative lymphocytes).
Expression of cell surface IL-12R 2 by T helper cells. Mouse Th1 cell line, 2D6 (left panel) and Th2 cell line, D10 (center panel) were stained with purified anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, 0.5 µg/test) followed by PE-conjugated anti-hamster IgG (0.25 µg, Cat . No. 554056). Staining with the HAM10B9 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The histograms in the figure were derived from gated events with the forward and side light scatter characteristics of viable lymphocytes. Mouse splenocytes from C57BL/6 mice (right panel) were treated with an ammonium chloride lysing buffer to remove the red blood cells. Cells were subsequently cultured with ConA (2 µg/ml), PMA (50 ng/ml), Dextran sulfate (10 µg/ml), LPS (5 µg/ml), recombinant mouse IL-2 (10 ng/ml), recombinant mouse IL-12p70 (20 ng/ml) and anti-IL-4 antibody, clone 11B11 (5 µg/ml) for 5 days. Following culture the cells were harvested, washed, blocked with mouse Fc Block™ (Cat. No. 553141) and stained with purified anti-mouse IL-12 receptor β2 antibody (clone HAM10B9, 0.5 µg/test) followed by PE-conjugated anti-hamster IgG (0.25 µg, Cat . No. 554056) and Viaprobe (Cat. No. 555816). Staining with anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, filled histograms) is compared to staining obtained using the isotype control antibody (Cat. No. 553969, open histograms). The histograms in the figure were derived from viable gated events (e.g. ViaProbe negative lymphocytes).
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