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BD Pharmingen- PE Mouse Anti-Human CD48_BD Pharmingen

艾美捷科技 • 2025年08月20日 14:20:37 • 抗体 • 阅读 34 • 标签
产品信息
荧光素标记
PE
抗原名称
CD48
宿主
Mouse IgM, κ
简单描述
The TÜ145 monoclonal antibody specifically binds to CD48. CD48 belongs to the CD2 family within the immunoglobulin superfamily. CD48 is a ~43 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD48 is strongly expressed on lymphocytes, monocytes and macrophages and weakly on granulocytes. The expression of CD48 is upregulated following lymphocyte activation and by Epstein-Barr virus (EBV) infection. CD48 binds with high affinity to CD244 and with low affinity to CD2. These receptor-ligand interactions can augment or inhibit leukocyte responses.
商品描述
TÜ145 The TÜ145 monoclonal antibody specifically binds to CD48. CD48 belongs to the CD2 family within the immunoglobulin superfamily. CD48 is a ~43 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD48 is strongly expressed on lymphocytes, monocytes and macrophages and weakly on granulocytes. The expression of CD48 is upregulated following lymphocyte activation and by Epstein-Barr virus (EBV) infection. CD48 binds with high affinity to CD244 and with low affinity to CD2. These receptor-ligand interactions can augment or inhibit leukocyte responses.
同种型
Mouse IgM, κ
克隆号
克隆 TÜ145 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD48
背景
别名
BCM1; BLAST; BLAST1; Hulym3; MEM-102; OX45; SLAMF2; TCT.1
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182. 2. Sandrin MS, Mouhtouris E, Vaughan HA, Warren HS, Parish CR. CD48 is a low affinity ligand for human CD2. J Immunol. 1993; 151(9):4606-4613. (Biology). 3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

参考图片

Flow cytometric analysis of CD48 expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD48 (Cat. No. 552855; solid line histogram) and PE Mouse IgM, κ Isotype Control (Cat. No.555584, dashed line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899).The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

Flow cytometric analysis of CD48 expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD48 (Cat. No. 552855; solid line histogram) and PE Mouse IgM, κ Isotype Control (Cat. No.555584, dashed line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899).The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

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