BD Pharmingen- Purified Rat Anti-Mouse VEGFR-3_BD Pharmingen
产品信息
抗原名称
VEGF Receptor 3 (FLT4)
宿主
Rat IgG2a, κ
免疫原
VEGFR-3 extracellular domain
简单描述
The AFL4 antibody reacts with mouse VEGFR-3, an endothelial-specific receptor tyrosine kinase (RTK) related to VEGF receptors. The immunogen used was the extracellular domain of VEGFR-3, corresponding to nucleotides 45-2354 in the GeneBank L07296. VEGFR-3 is induced in all endothelial cells (EC's) during early embryogenesis, and its expression eventually disappears from the vascular endothelial cells of adult tissues. VEGFR-3 is constitutively expressed in the adult lymphatic endothelium. Although VEGFR-3 is not expressed in adult blood vessels, it is induced in vascular endothelial cells of tumor-bearing tissues. Studies have shown that both VEGF-C and VEGF-D, ligands for VEGFR-3, promote lymphatic vessel proliferation throughout central tumor areas and at their peripheries, demonstrating that such molecules aid in the spread of tumor cells from the primary tumor to local lymph nodes.
商品描述
AFL4
The AFL4 antibody reacts with mouse VEGFR-3, an endothelial-specific receptor tyrosine kinase (RTK) related to VEGF receptors. The immunogen used was the extracellular domain of VEGFR-3, corresponding to nucleotides 45-2354 in the GeneBank L07296. VEGFR-3 is induced in all endothelial cells (EC's) during early embryogenesis, and its expression eventually disappears from the vascular endothelial cells of adult tissues. VEGFR-3 is constitutively expressed in the adult lymphatic endothelium. Although VEGFR-3 is not expressed in adult blood vessels, it is induced in vascular endothelial cells of tumor-bearing tissues. Studies have shown that both VEGF-C and VEGF-D, ligands for VEGFR-3, promote lymphatic vessel proliferation throughout central tumor areas and at their peripheries, demonstrating that such molecules aid in the spread of tumor cells from the primary tumor to local lymph nodes.
同种型
Rat IgG2a, κ
克隆号
克隆 AFL4 (RUO)
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Immunohistochemistry-paraffin (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
VEGF Receptor 3 (FLT4)
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Kubo H, Fujiwara T, Jussila L, et al. Involvement of vascular endothelial growth factor receptor-3 in maintenance of integrity of endothelial cell lining during tumor angiogenesis. Blood. 2000; 96(2):546-553. (Clone-specific: Western blot).
2. Stacker SA, Caesar C, Baldwin ME, et al. VEGF-D promotes the metastatic spread of tumor cells via the lymphatics. Nat Med. 2001; 7(2):186-191. (Biology).
参考图片
Immunohistochemical staining of mouse liver cells. Formalin-fixed, paraffin-embedded sections of Balb/c mouse liver were stained with VEGFR-3 antibody, clone AFL4 (Cat. No. 552857). The antibody labeled endothelial cells of sinusoid. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Magnification 40x.
Immunohistochemical staining of mouse liver cells. Formalin-fixed, paraffin-embedded sections of Balb/c mouse liver were stained with VEGFR-3 antibody, clone AFL4 (Cat. No. 552857). The antibody labeled endothelial cells of sinusoid. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) was performed prior to staining. Magnification 40x.
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