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BD Pharmingen- Purified Mouse Anti-Human CD314 -NKG2D_BD Pharmingen

艾美捷科技 • 2025年08月20日 14:23:21 • 抗体 • 阅读 8 • 标签
产品信息
抗原名称
CD314
宿主
Mouse RBF/ DnJ IgG1, κ
免疫原
NKL cells
简单描述
The 1D11 monoclonal antibody specifically binds to NKG2D, a 42 kDa type II transmembrane glycoprotein that is also known as CD314 and KLRK1. NKG2D is a member of the C-type lectin family and is expressed on human NK cells. This activating receptor binds strongly to several ligands including MICA and MICB and ULBP-1, -2, and -3 proteins that are expressed by different target cell types. Different from natural cytotoxicity receptor (NCR), NKG2D expression is not confined to NK cells. It is also expressed on virtually all TCR γ/δ+ and CD8+TCR α/β+ T cells. NKG2D functions as a triggering receptor involved in natural cytotoxicity mediated by normal NK cells against a variety of tumors or normal target cells. Importantly, NKG2D can complement the role of NCR in tumor cell lysis. Remarkably, the combined maskings of NCR and NKG2D can reportedly lead to a complete inhibition of NK-mediated lysis of all tumor or normal cells. The 1D11 antibody can reportedly block or stimulate the function of NKG2D-positive cells.
商品描述
1D11 The 1D11 monoclonal antibody specifically binds to NKG2D, a 42 kDa type II transmembrane glycoprotein that is also known as CD314 and KLRK1. NKG2D is a member of the C-type lectin family and is expressed on human NK cells. This activating receptor binds strongly to several ligands including MICA and MICB and ULBP-1, -2, and -3 proteins that are expressed by different target cell types. Different from natural cytotoxicity receptor (NCR), NKG2D expression is not confined to NK cells. It is also expressed on virtually all TCR γ/δ+ and CD8+TCR α/β+ T cells. NKG2D functions as a triggering receptor involved in natural cytotoxicity mediated by normal NK cells against a variety of tumors or normal target cells. Importantly, NKG2D can complement the role of NCR in tumor cell lysis. Remarkably, the combined maskings of NCR and NKG2D can reportedly lead to a complete inhibition of NK-mediated lysis of all tumor or normal cells. The 1D11 antibody can reportedly block or stimulate the function of NKG2D-positive cells.
同种型
Mouse RBF/ DnJ IgG1, κ
克隆号
克隆 1D11 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
CD314 (NKG2D)
背景
别名
CD314; KLRK1; KLR; NKG2D; NKG2-D; NK cell receptor D
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 1999; 285(5428):727-729. (Biology). 2. Groh V, Rhinehart R, Randolph-Habecker J, Topp MS, Riddell SR, Spies T. Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells. Nat Immunol. 2001; 2(3):255-260. (Biology).
数据库链接
Entrez-Gene ID
22914

参考图片

Flow cytometric analysis of NKG2D expression on human peripheral blood lymphocytes. Whole blood was stained with Purified Mouse Anti-Human NKG2D (Cat. No. 552866) and APC Mouse Anti-Human CD3ε (Cat. No. 558257). Secondary staining was carried out using FITC Goat Anti-Mouse IgG/IgM (Cat. No. 552866), and erythrocytes were lysed using BD FACS™ Lysing Solution (Cat. No. 349202). The two-color fluorescent dot plot was derived from gated events using the side and forward light-scatter characteristics of viable lymphocytes.

Flow cytometric analysis of NKG2D expression on human peripheral blood lymphocytes. Whole blood was stained with Purified Mouse Anti-Human NKG2D (Cat. No. 552866) and APC Mouse Anti-Human CD3ε (Cat. No. 558257). Secondary staining was carried out using FITC Goat Anti-Mouse IgG/IgM (Cat. No. 552866), and erythrocytes were lysed using BD FACS™ Lysing Solution (Cat. No. 349202). The two-color fluorescent dot plot was derived from gated events using the side and forward light-scatter characteristics of viable lymphocytes.

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