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BD Pharmingen- PE Rat Anti-Mouse Ly-6G and Ly-6C_BD Pharmingen

艾美捷科技 • 2025年08月20日 16:15:54 • 抗体 • 阅读 47 • 标签
产品信息
荧光素标记
PE
抗原名称
Ly-6G/Ly-6C
宿主
Rat IgG2b, κ
免疫原
Not Reported
简单描述
The RB6-8C5 monoclonal antibody recognizes a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 antibody is a component of the "lineage cocktail" used in studies of hematopoietic cell lineages. The 1A8 antibody (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. Based on comparison of the staining patterns given by 1A8 versus RB6-8C5 antibodies on total blood leucocytes, it is evident that the 1A8 antibody stains the RB6-8C5-bright population, corresponding to Ly-6G-expressing granulocytes; whereas, the RB6-8C5-dim population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Please refer to the Technical Data Sheets for Cat. No. 551459 and 553128 for more detailed information.
商品描述
RB6-8C5 The RB6-8C5 monoclonal antibody recognizes a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 antibody is a component of the "lineage cocktail" used in studies of hematopoietic cell lineages. The 1A8 antibody (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. Based on comparison of the staining patterns given by 1A8 versus RB6-8C5 antibodies on total blood leucocytes, it is evident that the 1A8 antibody stains the RB6-8C5-bright population, corresponding to Ly-6G-expressing granulocytes; whereas, the RB6-8C5-dim population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Please refer to the Technical Data Sheets for Cat. No. 551459 and 553128 for more detailed information.
同种型
Rat IgG2b, κ
克隆号
克隆 RB6-8C5 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
Ly-6G/Ly-6C
背景
别名
Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(8) 1. Conlan JW, North RJ. Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody. J Exp Med. 1994; 179(1):259-268. (Clone-specific: Depletion). 2. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Clone-specific: Immunoprecipitation, Inhibition). 3. Hestdal K, Ruscetti FW, Ihle JN, et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991; 147(1):22-28. (Biology). 4. Jutila MA, Kroese FG, Jutila KL, et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. Eur J Immunol. 1988; 18(11):1819-1826. (Clone-specific: Western blot). 5. Lagasse E, Weissman IL. Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods. 1996; 197(1-2):139-150. (Biology). 6. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5. 7. Stoppacciaro A, Melani C, Parenza M, et al. Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma. J Exp Med. 1993; 178(1):151-161. (Clone-specific: Depletion, Immunohistochemistry). 8. Tepper RI, Coffman RL, Leder P. An eosinophil-dependent mechanism for the antitumor effect of interleukin-4. Science. 1992; 257(5069):548-551. (Clone-specific: Depletion).

参考图片

Representative staining of peripheral blood leukocytes with PE-conjugated antibody RB6-8C5. C57BL/6 whole blood was stained with PE-conjugated RB6-8C5 (anti-Ly-6G and Ly-6C) and FITC-conjugated 1A8 (anti-mouse Ly-6G, Cat. No. 551460) monoclonal antibodies in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142, left panel). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899) and non-viable leukocytes were excluded by staining with propidium iodide. The left panel demonstrates that mAb 1A8 stains the RB6-8C5hi population, corresponding to Ly-6G-expressing granulocytes; whereas, the RB6-8C5lo population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Backgating of the RB6-8C5+/1A8- population (R1) onto the light-scatter profile (right panel) indicates that this population falls within the monocyte region of the light-scatter profile. The RB6-8C5+/1A8+ population (R2) falls within the granulocyte region of the light-scatter profile (right panel). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Representative staining of peripheral blood leukocytes with PE-conjugated antibody RB6-8C5. C57BL/6 whole blood was stained with PE-conjugated RB6-8C5 (anti-Ly-6G and Ly-6C) and FITC-conjugated 1A8 (anti-mouse Ly-6G, Cat. No. 551460) monoclonal antibodies in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142, left panel). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899) and non-viable leukocytes were excluded by staining with propidium iodide. The left panel demonstrates that mAb 1A8 stains the RB6-8C5hi population, corresponding to Ly-6G-expressing granulocytes; whereas,  the RB6-8C5lo population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Backgating of the RB6-8C5+/1A8- population (R1) onto the light-scatter profile (right panel) indicates that this population falls within the monocyte region of the light-scatter profile. The RB6-8C5+/1A8+ population (R2) falls within the granulocyte region of the light-scatter profile (right panel). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

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