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BD Pharmingen- PE-Cy-5 Hamster Anti-Mouse TCR - Chain_BD Pharmingen

艾美捷科技 • 2025年08月20日 16:47:52 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
PE-Cy5
抗原名称
TCR β Chain
宿主
Armenian Hamster IgG2, λ1
免疫原
TCR affinity-purified from mouse T-cell hybridoma DO-11.10
简单描述
The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.
商品描述
H57-597 The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.
同种型
Armenian Hamster IgG2, λ1
克隆号
克隆 H57-597 (RUO)
浓度
0.2 mg/ml
产品详情
PE-Cy5
PE-Cy5 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496 nm and 566 nm and an acceptor dye, Cy™5, with an emission maximum (Em Max) at 670-nm. PE designed to be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 670-nm (e.g., a 670/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627-640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE-Cy5
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
670 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR β Chain
背景
别名
Tcrb; TCRbeta; TCRβ, T cell receptor beta chain
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(8) 1. Duke RC, Cohen JJ, Boehme SA, et al. Morphological, biochemical, and flow cytometric assays of apoptosis. In: Coligan J, Kruisbeek AM, Margulies D, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1995:3.17.1-3.17.33. 2. Gascoigne NR. Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990; 265(16):9296-9301. (Clone-specific: Immunoprecipitation). 3. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Stimulation). 4. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Immunogen: Flow cytometry). 5. Saint-Ruf C, Ungewiss K, Groettrup M, Bruno L, Fehling HJ, von Boehmer H. Analysis and expression of a cloned pre-T cell receptor gene. Science. 1994; 266(5188):1208-1212. (Clone-specific: Stimulation). 6. Takizawa F, Kinet JP, Adamczewski M. Binding of phycoerythrin and its conjugates to murine low affinity receptors for immunoglobulin G. J Immunol Methods. 1993; 162(2):269-272. (Biology). 7. Waggoner AS, Ernst LA, Chen CH, Rechtenwald DJ. PE-CY5. A new fluorescent antibody label for three-color flow cytometry with a single laser. Ann N Y Acad Sci. 1993; 677:185-193. (Clone-specific: Cytotoxicity). 8. van Vugt MJ, van den Herik-Oudijk IE, van de Winkle JG. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996; 88(6):2358-2361. (Biology).
数据库链接
Entrez-Gene ID
21577

参考图片

αβ TCR expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553048/553049, left panels), PE-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553032/553033, left panels), and PE-Cy5-conjugated mAb H57-597 (bottom left panel) monoclonal antibodies, in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142, left panels). BALB/c thymocytes were stained with PE-Cy5-conjugated mAb H57-597 (bottom right panel) or unstained (top right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Flow cytometric analysis of αβ TCR expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 (Cat. No. 553048/553049; left panels), PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033; left panels), and PE-Cy™5 Hamster Anti-Mouse TCR β Chain (Cat. No. 553173; bottom left panel) monoclonal antibodies, in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), (Cat. No. 553141/553142; left panels). BALB/c thymocytes were stained with PE-Cy™5 Hamster Anti-Mouse TCR β Chain (bottom right panel) or unstained (top right panel). The fluorescence histograms and contour plots were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.

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