BD Pharmingen- FITC Mouse Anti-Mouse V- 8-1- 8-2 TCR_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
TCR Vβ8.1, 8.2
宿主
Mouse C57L IgG2a, κ
免疫原
C57BL/6 mouse helper T-cell clone OI6
简单描述
The MR5-2 antibody reacts with the Vβ 8.1 and Vβ 8.2 T-cell Receptors (TCR), but not the Vβ 8.3 TCR, of mice having the
b
haplotype (
e.g
., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the
Tcrb
gene complex. The
Tcrb-V8
subfamily gene loci are deleted in mice having the
a
(
e.g
., C57BR, C57L, SJL, SWR) or
c
(
e.g.,
RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by the
Mtv-7 (Mls-1a, Mlsa),
provirus (
e.g.,
AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.
Mtv-43
(e.g., MA/MyJ),
Mtv-44 (e.g.,
NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells
in vivo
. Plate-bound MR5-2 antibody activates Vβ 8.1 or 8.2 TCR-bearing T lymphocytes.
商品描述
MR5-2
The MR5-2 antibody reacts with the Vβ 8.1 and Vβ 8.2 T-cell Receptors (TCR), but not the Vβ 8.3 TCR, of mice having the
b
haplotype (
e.g
., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the
Tcrb
gene complex. The
Tcrb-V8
subfamily gene loci are deleted in mice having the
a
(
e.g
., C57BR, C57L, SJL, SWR) or
c
(
e.g.,
RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by the
Mtv-7 (Mls-1a, Mlsa),
provirus (
e.g.,
AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.
Mtv-43
(e.g., MA/MyJ),
Mtv-44 (e.g.,
NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells
in vivo
. Plate-bound MR5-2 antibody activates Vβ 8.1 or 8.2 TCR-bearing T lymphocytes.
同种型
Mouse C57L IgG2a, κ
克隆号
克隆 MR5-2 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ8.1, 8.2
背景
别名
TCR V beta 8.1; TCR V beta 8.2
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(11)
1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology).
2. Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology).
3. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology).
4. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology).
5. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology).
6. Hugo P, Kappler JW, Godfrey DI, Marrack PC. Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion. J Immunol. 1994; 52(3):1022-1031. (Biology).
7. Kanagawa O. Antibody-mediated activation of T cell clones as a method for screening hybridomas producing antibodies to the T cell receptor. J Immunol Methods. 1988; 110(2):169-178. (Immunogen).
8. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Biology).
9. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology).
10. Tomonari K, Fairchild S. Positive and negative selection of Tcrb-V6+ T cells. Immunogenetics. 1992; 36(4):230-237. (Biology).
11. White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. (Biology).
参考图片
Two-color analysis of the expression of Vβ 8.1, 8.2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Mouse Anti-Mouse Vβ 8.1, 8.2 TCR (Cat. No. 553185), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Two-color analysis of the expression of Vβ 8.1, 8.2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Mouse Anti-Mouse Vβ 8.1, 8.2 TCR (Cat. No. 553185), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
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