BD Pharmingen- FITC Mouse Anti-Mouse V-9 TCR_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
TCR Vβ9
宿主
Mouse SWR IgG1, κ
免疫原
Mouse T-cell hybridoma 2BR18
简单描述
The MR10-2 monoclonal antibody reacts with the Vβ9 T-cell Receptor (TCR) of mice having the
b
haplotype (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL) of the
Tcrb
gene complex. The Tcrb-V9 gene locus is deleted in mice having the
a
(
e.g.,
C57BR, C57L, SJL, SWR) or
c
(
e.g
., RIII) haplotype. Vβ9-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by
Mtv-7 (Mls-1a, Mlsa)
provirus (
e.g.,
AKR, CBA/J, DBA/2), and activation or elimination of Vβ9 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.
Mtv-43 (e.g
., MA/MyJ),
Mtv-44 (e.g
., NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ9 TCR-bearing T cells. Plate-bound MR10-2 antibody activates Vβ9 TCR-bearing T cells.
商品描述
MR10-2
The MR10-2 monoclonal antibody reacts with the Vβ9 T-cell Receptor (TCR) of mice having the
b
haplotype (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL) of the
Tcrb
gene complex. The Tcrb-V9 gene locus is deleted in mice having the
a
(
e.g.,
C57BR, C57L, SJL, SWR) or
c
(
e.g
., RIII) haplotype. Vβ9-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by
Mtv-7 (Mls-1a, Mlsa)
provirus (
e.g.,
AKR, CBA/J, DBA/2), and activation or elimination of Vβ9 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.
Mtv-43 (e.g
., MA/MyJ),
Mtv-44 (e.g
., NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ9 TCR-bearing T cells. Plate-bound MR10-2 antibody activates Vβ9 TCR-bearing T cells.
同种型
Mouse SWR IgG1, κ
克隆号
克隆 MR10-2 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ9
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6)
1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology).
2. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology).
3. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology).
4. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology).
5. Tomonari K, Fairchild S. Positive and negative selection of Tcrb-V6+ T cells. Immunogenetics. 1992; 36(4):230-237. (Biology).
6. Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991; 21(4):1007-1011. (Immunogen).
参考图片
Two-color analysis of the expression of Vβ 9 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated MR10-2, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
Two-color analysis of the expression of Vβ 9 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated MR10-2, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
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