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BD Pharmingen- FITC Mouse Anti-Mouse V- 13 TCR_BD Pharmingen

艾美捷科技 • 2025年08月20日 17:02:21 • 抗体 • 阅读 8 • 标签
产品信息
荧光素标记
FITC
抗原名称
TCR Vβ13
宿主
Mouse C57BR IgG1, κ
免疫原
Not reported
简单描述
The MR12-3 monoclonal antibody specifically binds to the Vβ 13 T-cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V13 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype.
商品描述
MR12-3 The MR12-3 monoclonal antibody specifically binds to the Vβ 13 T-cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V13 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype.
同种型
Mouse C57BR IgG1, κ
克隆号
克隆 MR12-3 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ13
背景
别名
TCR V beta 13; TCR Vβ 13; TCR Vβ13; Vβ 13 T-cell Receptor
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). 2. Chen D, Lee F, Cebra JJ, Rubin DH. Predominant T-cell receptor Vbeta usage of intraepithelial lymphocytes during the immune response to enteric reovirus infection.. J Virol. 1997; 71(5):3431-6. (Biology). 3. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology).

参考图片

Flow cytometric analysis of Vβ 13 TCR expression on mouse peripheral blood lymphocytes. C57BL/6 lymph node cells simultaneously stained with FITC Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 553204), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033). The two-color contour plot depicting Vβ 13 TCR versus CD4 and CD8a expression was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

Flow cytometric analysis of Vβ 13 TCR expression on mouse peripheral blood lymphocytes. C57BL/6 lymph node cells simultaneously stained with FITC Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 553204), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033). The two-color contour plot depicting Vβ 13 TCR versus CD4 and CD8a expression was derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

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