BD Pharmingen- FITC Rat Anti-Mouse V- 3-2-b-c- TCR_BD Pharmingen
产品信息
荧光素标记
FITC
抗原名称
TCR Vα3.2[b,c]
宿主
Rat F344, also known as Fischer, CDF IgG2b, κ
免疫原
Mouse Cytolytic T-Cell Clone OH6
简单描述
The RR3-16 monoclonal antibody specifically reacts with the Vα 3.2 T-Cell Receptor (TCR) of mice having the
b
(e.g., C57BL) and
c
(e.g., SWR, SJL, NZB, NOD) haplotypes of the
Tcra
gene complex, but not with TCR encoded by other members of
Tcra-V3
gene subfamily. RR3-16 antibody does not react with strains having the
a
(e.g., A, AKR, BALB/c, CBA, C3H/He) or
d
(e.g., DBA/1, DBA/2, NZW)
Tcra
haplotypes. In addition, it has been shown that frequencies of Vα 3.2+ CD8+ T cells from homozygous H-2k/H-2k mice are moderately higher than those from heterozygous H-2k/H-2d mice, suggesting positive selection by H-2k.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
RR3-16
The RR3-16 monoclonal antibody specifically reacts with the Vα 3.2 T-Cell Receptor (TCR) of mice having the
b
(e.g., C57BL) and
c
(e.g., SWR, SJL, NZB, NOD) haplotypes of the
Tcra
gene complex, but not with TCR encoded by other members of
Tcra-V3
gene subfamily. RR3-16 antibody does not react with strains having the
a
(e.g., A, AKR, BALB/c, CBA, C3H/He) or
d
(e.g., DBA/1, DBA/2, NZW)
Tcra
haplotypes. In addition, it has been shown that frequencies of Vα 3.2+ CD8+ T cells from homozygous H-2k/H-2k mice are moderately higher than those from heterozygous H-2k/H-2d mice, suggesting positive selection by H-2k.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat F344, also known as Fischer, CDF IgG2b, κ
克隆号
克隆 RR3-16 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vα3.2[b,c]
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology).
2. Utsunomiya Y, Bill J, Palmer E, Gollob K, Takagaki Y, Kanagawa O. Analysis of a monoclonal rat antibody directed to the alpha-chain variable region (V alpha 3) of the mouse T cell antigen receptor. J Immunol. 1989; 143(8):2602-2608. (Immunogen).
3. Utsunomiya Y, Bill J, Palmer E, Kanagawa O. Identification of a mouse T-cell antigen receptor alpha-chain polymorphism by a V alpha 3.2 chain-specific monoclonal antibody. Immunogenetics. 1991; 33(3):198-201. (Clone-specific).
参考图片
Two-color analysis of the expression of Vα 3.2b,c TCR on peripheral lymphocytes. C57BL/6 lymph node leukocytes were simultaneuosly stained with FITC-conjugated RR3-16, PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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