BD Pharmingen- PE Rat Anti-Mouse V- 7 T-Cell Receptor_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
TCR Vβ7
宿主
Rat F344, also known as Fischer, CDF IgG2b, κ
免疫原
AKR/Cum Mouse spontaneous T Lymphoma Cell Line TK1
简单描述
The TR310 monoclonal antibody specifically recognizes the Vβ 7 T-Cell Receptor (TCR) of strains having the
a
(e.g.,
C57BR, C57L, SJL),
b
(e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1), and
c
(e.g., RIII) haplotypes of the
Tcrb
gene complex. Vβ 7 is among the predominant TCRs expressed by NK-T cells of C57BL/6 mice. Vβ 7 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigens encoded by
Mtv-7
(
Mls-1[a], Mls[a]
) endogenous proviruses (e.g., AKR, CBA/J, C58, DBA/2, NZB). Activation or elimination of Vβ 7 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E.
Mtv-43
and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 7 TCR-bearing T cells.
商品描述
TR310
The TR310 monoclonal antibody specifically recognizes the Vβ 7 T-Cell Receptor (TCR) of strains having the
a
(e.g.,
C57BR, C57L, SJL),
b
(e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1), and
c
(e.g., RIII) haplotypes of the
Tcrb
gene complex. Vβ 7 is among the predominant TCRs expressed by NK-T cells of C57BL/6 mice. Vβ 7 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigens encoded by
Mtv-7
(
Mls-1[a], Mls[a]
) endogenous proviruses (e.g., AKR, CBA/J, C58, DBA/2, NZB). Activation or elimination of Vβ 7 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E.
Mtv-43
and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 7 TCR-bearing T cells.
同种型
Rat F344, also known as Fischer, CDF IgG2b, κ
克隆号
克隆 TR310 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ7
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology).
2. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology).
3. Okada CY, Holzmann B, Guidos C, Palmer E, Weissman IL. Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype. J Immunol. 1990; 144(9):3473-3477. (Immunogen).
4. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology).
参考图片
Two-color analysis of the expression of Vβ 7 TCR on peripheral T lymphocytes. BALB/c lymph node cells were simultaneously stained with PE-conjugated TR310, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
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