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BD Pharmingen- FITC Rat Anti-Mouse V- 10-b- TCR_BD Pharmingen

产品信息
荧光素标记
FITC
抗原名称
TCR Vβ10[b]
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
免疫原
Mouse Cytotoxic T-Cell Clone Soluble αβ TCR
简单描述
The B21.5 monoclonal antibody specifically recognizes the Vβ 10 T-Cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. It does not react with mice having the a (eg, C57BR, C57L, SJL, SWR) or c (eg, RIII) Tcrb haplotype.
商品描述
B21.5 The B21.5 monoclonal antibody specifically recognizes the Vβ 10 T-Cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. It does not react with mice having the a (eg, C57BR, C57L, SJL, SWR) or c (eg, RIII) Tcrb haplotype.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
克隆号
克隆 B21.5 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ10[b]
背景
别名
TCR Vβ10; TCR V beta 10; TCR Vb10
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1) 1. Necker A, Rebai N, Matthes M, et al. Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V alpha 8-, V beta 2- or V beta 10-bearing T cells. Eur J Immunol. 1991; 21(12):3035-3040. (Immunogen).

参考图片

Two-color analysis of the expression of Vβ 10[b] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Rat Anti-Mouse Vβ 10[b] TCR (Cat. No.553284), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plots were derived from gated events based on the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™.

Two-color analysis of the expression of Vβ 10[b] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Rat Anti-Mouse Vβ 10[b] TCR (Cat. No.553284), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plots were derived from gated events based on the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™.

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