1. 首页
  2. 抗体
  3. 正文

BD Pharmingen- FITC Rat Anti-Mouse V-2 TCR_BD Pharmingen

艾美捷科技 • 2025年08月21日 09:36:01 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
FITC
抗原名称
TCR Vα2
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
免疫原
Soluble αβ TCR from mouse cytotoxic T-cell clone KB5-C20
简单描述
The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes. For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
商品描述
B20.1 The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes. For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
克隆号
克隆 B20.1 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vα2
背景
别名
T cell receptor alpha, variable region 2
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Clone-specific). 2. Pircher H, Rebaï N, Groettrup M, et al. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.. Eur J Immunol. 1992; 22(2):399-404. (Immunogen). 3. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology).
数据库链接
Entrez-Gene ID
111660

参考图片

Flow cytometric analysis of Vα2 TCR expression on mice peripheral lymphocytes. BALB/c lymph node cells were incubated simultaneously with PE Rat Anti-Mouse CD8b.2 (Cat. No. 553041), PE Rat Anti-Mouse CD4 (Cat. No. 553730/561829/557308) and FITC Rat Anti-Mouse Vα2 TCR (Cat. No. 553288/562085). The contour plot depicting CD4 versus Vα2 TCR expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™ system.

Flow cytometric analysis of Vα2 TCR expression on mice peripheral lymphocytes. BALB/c lymph node cells were incubated simultaneously with PE Rat Anti-Mouse CD8b.2  (Cat. No. 553041), PE Rat Anti-Mouse CD4 (Cat. No. 553730/561829/557308) and FITC Rat Anti-Mouse Vα2 TCR (Cat. No. 553288/562085). The contour plot depicting CD4 versus Vα2 TCR expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™ system.

文档下载: W 导出为BD Pharmingen- FITC Rat Anti-Mouse V-2 TCR_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9266.html

赞 (7)
打赏 微信扫一扫
« 上一篇 2025年08月21日 09:35:06
下一篇 » 2025年08月21日 09:37:33

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();