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BD Pharmingen- Biotin Rat Anti-Mouse V-4 T-Cell Receptor_BD Pharmingen

艾美捷科技 • 2025年08月21日 10:09:47 • 抗体 • 阅读 22 • 标签
产品信息
抗原名称
TCR Vβ4
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
免疫原
KT4 B10.D2 mouse T-cell clone I3
简单描述
The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a ( e.g ., C57BR, C57L, SJL, SWR), b ( e.g ., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c ( e.g ., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.
商品描述
KT4 The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a ( e.g ., C57BR, C57L, SJL, SWR), b ( e.g ., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c ( e.g ., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
克隆号
克隆 KT4 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ4
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Katz JD, Benoist C, Mathis D. T helper cell subsets in insulin-dependent diabetes. Science. 1995; 268(5214):1185-1188. (Biology). 2. Sugihara S, Fujiwara H, Shearer GM. Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions. J Immunol. 1993; 150(2):683-694. (Biology). 3. Tomonari K, Lovering E, Spencer S. Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype. Immunogenetics. 1990; 31(5-6):333-339. (Immunogen).

参考图片

Two-color analysis of the expression of Vb 4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with biotinylated KT4 (Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

Two-color analysis of the expression of Vb 4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with biotinylated KT4 (Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

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