BD Pharmingen- Purified Rat Anti-Mouse IgG2a_BD Pharmingen
产品信息
抗原名称
IgG2a
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled BALB/c and C57BL/6 mouse Ig
简单描述
The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the
Igh-I[a]
allotype and weaker reactivity to
Igh-I[b]
. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the
Igh-I[b]
allele, which encodes
IgG2a[b]
,
is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
This antibody is routinely tested by ELISA analysis. Other applications were tested at BD BIosciences Pharmingen during antibody development only or reported in the literature.
商品描述
R19-15
The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the
Igh-I[a]
allotype and weaker reactivity to
Igh-I[b]
. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the
Igh-I[b]
allele, which encodes
IgG2a[b]
,
is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
This antibody is routinely tested by ELISA analysis. Other applications were tested at BD BIosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 R19-15 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgG2a
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology).
2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology).
数据库链接
Entrez-Gene ID
380793
参考图片
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