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BD Pharmingen- FITC Anti-Mouse IgG2a_BD Pharmingen

艾美捷科技 • 2025年08月21日 10:19:46 • 抗体 • 阅读 88 • 标签
产品信息
荧光素标记
FITC
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled BALB/c and C57BL/6 mouse Ig
简单描述
The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b] . It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b] ,   is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
商品描述
R19-15 The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b] . It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b] , is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 R19-15 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
IgG2a
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). 2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology).
数据库链接
Entrez-Gene ID
380793

参考图片

Detection of intracellular mouse IgG2a in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained with either the FITC anti-Mouse IgG2a mAb (Clone R19-15, filled histogram) or the matched FITC Rat IgG1, κ Isotype Control (Open histogram, Cat. No. 554684/553924). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Detection of intracellular mouse IgG2a in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained with either the FITC anti-Mouse IgG2a mAb (Clone R19-15, filled histogram) or the matched FITC Rat IgG1, κ Isotype Control (Open histogram, Cat. No. 554684/553924). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

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