BD Pharmingen- FITC Rat Anti-Mouse Ig- -1- -2- - -3 Light Chain_BD Pharmingen
产品信息
荧光素标记
FITC
宿主
Rat IgG2a, κ
免疫原
Pooled Mouse Ig
简单描述
The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.
商品描述
R26-46
The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.
同种型
Rat IgG2a, κ
克隆号
克隆 R26-46 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
Ig, λ1, λ2, λ3 Light Chain
背景
别名
Igl; Ig λ; Immunoglobulin lambda chain complex; Ig λ1/λ2/λ3
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors.. J Exp Med. 2002; 195(2):181-8. (Clone-specific).
2. Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.. J Exp Med. 2000; 191(1):23-32. (Clone-specific).
数据库链接
Entrez-Gene ID
111519
参考图片
Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
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