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BD Pharmingen- FITC Rat Anti-Mouse IgM_BD Pharmingen

艾美捷科技 • 2025年08月21日 10:41:47 • 抗体 • 阅读 55 • 标签
产品信息
荧光素标记
FITC
抗原名称
IgM
宿主
Rat IgG2a, κ
免疫原
Not reported
简单描述
The II/41 clone has been reported to react specifically with mouse IgM of Igh-C[a] and Igh-C[b] haplotypes. It has been reported not to react with other Ig isotypes. In addition, the II/41 clone has been reported not to stimulate B-cell proliferation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
II/41 The II/41 clone has been reported to react specifically with mouse IgM of Igh-C[a] and Igh-C[b] haplotypes. It has been reported not to react with other Ig isotypes. In addition, the II/41 clone has been reported not to stimulate B-cell proliferation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG2a, κ
克隆号
克隆 II/41 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
IgM
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1) 1. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Biology).
数据库链接
Entrez-Gene ID
16019

参考图片

Detection of intracellular mouse IgM in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described in the recommended assay procedure using FITC-conjugated rat anti-mouse IgM (clone II/41) (filled histogram, Cat. No. 553437) or the matched isotype control, FITC rat IgG2a (clone R35-95) (open histogram, Cat. No. 554688). Flow cytometry was performed on a BD FACSCalibur™ instrument.

Detection of intracellular mouse IgM in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described in the recommended assay procedure using FITC-conjugated rat anti-mouse IgM (clone II/41) (filled histogram, Cat. No. 553437) or the matched isotype control, FITC rat IgG2a (clone R35-95) (open histogram, Cat. No. 554688).  Flow cytometry was performed on a BD FACSCalibur™ instrument.

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