BD Pharmingen- PE Mouse IgG2a- - Isotype Control

BD Pharmingen- PE Mouse IgG2a- - Isotype Control_BD Pharmingen

艾美捷科技 • 2025年08月21日 10:50:55 • 抗体 • 阅读 9 • 标签

产品信息

荧光素标记

免疫原

TNP-keyhole limpet hemocyanin

简单描述

The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.

商品描述

G155-178 The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.

同种型

Mouse BALB/c IgG2a, κ

克隆号

克隆 G155-178 (RUO)

浓度

0.2 mg/ml

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)

目标/特异性

IgG2a isotype control

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(1) 1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Blocking, Flow cytometry).

BD Pharmingen- PE Mouse IgG2a- - Isotype Control_BD Pharmingen

相关推荐