BD Pharmingen- PE Mouse Anti-Mouse V- 8 T-Cell Receptor_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
TCR Vβ8
宿主
Mouse C57L IgG2a, κ
免疫原
BALB.B Mouse Lymph-Node and Spleen T Cells
简单描述
The F23.1 antibody specifically reacts with the Vβ 8.1, Vβ 8.2, and Vβ 8.3 T-cell receptors (TCR) of mice having the
b
haplotype (
e.g.,
A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the
Tcrb
gene complex. The
Tcrb-V8
subfamily gene loci are deleted in mice having the
a
(
e.g.,
C57BR, C57L, SJL, SWR) or c (
e.g.,
RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by
Mtv-7 (Mls-1a, Mlsa)
provirus (
e.g.,
AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.
Mtv-43
and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells
in vivo
. Soluble and plate-bound F23.1 antibody activates Vβ 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by Vβ 8 TCR-bearing cytotoxic T lymphocytes, and
in vivo
treatment of neonatal mice can arrest intrathymic maturation of Vβ 8 TCR-bearing T cells.
商品描述
F23.1
The F23.1 antibody specifically reacts with the Vβ 8.1, Vβ 8.2, and Vβ 8.3 T-cell receptors (TCR) of mice having the
b
haplotype (
e.g.,
A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the
Tcrb
gene complex. The
Tcrb-V8
subfamily gene loci are deleted in mice having the
a
(
e.g.,
C57BR, C57L, SJL, SWR) or c (
e.g.,
RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by
Mtv-7 (Mls-1a, Mlsa)
provirus (
e.g.,
AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.
Mtv-43
and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells
in vivo
. Soluble and plate-bound F23.1 antibody activates Vβ 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by Vβ 8 TCR-bearing cytotoxic T lymphocytes, and
in vivo
treatment of neonatal mice can arrest intrathymic maturation of Vβ 8 TCR-bearing T cells.
同种型
Mouse C57L IgG2a, κ
克隆号
克隆 F23.1 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ8
背景
别名
TCR V beta 8; TCR V beta 8.1/8.2/8.3
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(15)
1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology).
2. Behlke MA, Henkel TJ, Anderson SJ, et al. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily. J Exp Med. 1987; 165(1):257-262. (Clone-specific).
3. Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology).
4. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology).
5. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology).
6. Hugo P, Kappler JW, Godfrey DI, Marrack PC. Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion. J Immunol. 1994; 52(3):1022-1031. (Biology).
7. Kappler JW, Staerz U, White J, Marrack PC. Self-tolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex. Nature. 1988; 332(6159):35-40. (Biology).
8. Kyewski BA, Schirrmacher V, Allison JP. Antibodies against the T cell receptor/CD3 complex interfere with distinct intra-thymic cell-cell interactions in vivo: correlation with arrest of T cell differentiation. Eur J Immunol. 1989; 19(5):857-863. (Clone-specific).
9. MacDonald HR, Baschieri S, Lees RK. Clonal expansion precedes anergy and death of V beta 8+ peripheral T cells responding to staphylococcal enterotoxin B in vivo. Eur J Immunol. 1991; 21(8):1963-1966. (Biology).
10. Mogil RJ, Radvanyi L, Gonzalez-Quintial R, et al. Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo. Int Immunol. 1995; 7(9):1451-1458. (Biology).
11. Renno T, Hahne M, Tschopp J, MacDonald HR. Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand. J Exp Med. 1996; 183(2):431-437. (Biology).
12. Staerz UD, Rammensee HG, Benedetto JD, Bevan MJ. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor. J Immunol. 1985; 134(6):3994-4000. (Immunogen).
13. White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. (Biology).
14. Wolff CH, Hong SC, von Grafenstein H, Janeway CA Jr. TCR-CD4 and TCR-TCR interactions as distinctive mechanisms for the induction of increased intracellular calcium in T-cell signalling. J Immunol. 1993; 151(3):1337-1345. (Clone-specific).
15. Yagi J, Nakata M, Uchiyama T, et al. Superantigen-like properties of an antibody bispecific for MHC class II molecules and the V beta domain of the T cell antigen receptor. J Immunol. 1994; 152(8):3833-3841. (Clone-specific).
参考图片
Two-color analysis of the expression of Vβ 8 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Mouse Anti-Mouse Vβ 8 T-Cell Receptor (Cat. No 553862) FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047), and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.
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