PNAd

Rat WF, also known as Wistar Furth IgM, κ

Collagenase-dispersed BALB/c lymph node stroma

The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of  peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin ( eg , CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo , and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.

MECA-79 The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of  peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin ( eg , CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo , and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.

Rat WF, also known as Wistar Furth IgM, κ

克隆 MECA-79 (RUO)

0.5 mg/ml

Immunohistochemistry-paraffin (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Immunoprecipitation, Western blot (Reported)

Mouse (QC Testing)

PNAd

CD62L Ligand

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(9) 1. Berg EL, Robinson MK, Warnock RA, Butcher EC. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J Cell Biol. 1991; 114(2):343-349. (Clone-specific: Blocking, Immunoprecipitation). 2. Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Biology). 3. Diacovo TG, Puri KD, Warnock RA, Springer TA, von Andrian UH. Platelet-mediated lymphocyte delivery to high endothelial venules. Science. 1996; 273(5272):252-255. (Clone-specific: Blocking). 4. Faveeuw C, Gagnerault MC, Lepault F. Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice. J Immunol. 1994; 152(12):5969-5978. (Biology). 5. Hemmerich S, Butcher EC, Rosen SD. Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J Exp Med. 1994; 180(6):2219-2226. (Clone-specific: Immunoprecipitation). 6. Malý P, Thall A, Petryniak B, et al. The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis. Cell. 1996; 86(4):643-653. (Biology). 7. Michie SA, Streeter PR, Bolt PA, Butcher EC, Picker LJ. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am J Pathol. 1993; 143(6):1688-1698. (Clone-specific: Blocking). 8. Puri KD, Finger EB, Gaudernack G, Springer TA. Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules. J Cell Biol. 1995; 131(1):261-270. (Clone-specific: Blocking, Western blot). 9. Streeter PR, Rouse BT, Butcher EC. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol. 1988; 107(5):1853-1862. (Immunogen: Blocking, Immunohistochemistry).