BD Pharmingen- FITC Mouse Anti-Rat IgG2a_BD Pharmingen
产品信息
荧光素标记
FITC
宿主
Mouse SJL IgG2b, κ
免疫原
Rat Pooled IgG
简单描述
The Rg7/1.30 antibody reacts specifically with the Fc region of rat IgG2a. It does not react with other Ig isotypes.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
RG7/1.30
The Rg7/1.30 antibody reacts specifically with the Fc region of rat IgG2a. It does not react with other Ig isotypes.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Mouse SJL IgG2b, κ
克隆号
克隆 RG7/1.30 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
反应种属
Rat (QC Testing)
目标/特异性
IgG2a
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen).
参考图片
Detection of intracellular rat IgG2a in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described below using FITC-conjugated RG7/1.30 mAb (filled histogram) or the matched isotype control, FITC-conjugated 27-35 mAb (open histogram, Cat. No. 555057). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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