BD Pharmingen- FITC Mouse Anti-Rat IgG2b_BD Pharmingen
产品信息
荧光素标记
FITC
宿主
Mouse SJL IgG2b, κ
免疫原
Pooled rat IgG
简单描述
The RG7/11.1 antibody reacts specifically with the Fc region of rat IgG2b. It does not react with other Ig isotypes.
商品描述
RG7/11.1
The RG7/11.1 antibody reacts specifically with the Fc region of rat IgG2b. It does not react with other Ig isotypes.
同种型
Mouse SJL IgG2b, κ
克隆号
克隆 RG7/11.1 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry, Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Rat (QC Testing)
目标/特异性
IgG2b
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen).
参考图片
Detection of intracellular rat IgG2b in an antibody-secreting hybidoma cell line. Cells were fixed, permeabilized, and stained according to the method described in the recommended assay procedure using FITC-conjugated RG7/11.1 mAb (filled histogram) or the matched isotype control, FITC-conjugated 27-35 mAb (open histogram, Cat. No. 555057). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9458.html