产品信息
荧光素标记
PerCP
抗原名称
Streptavidin
简单描述
SAv-PerCP is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.
PerCP is a photosynthetic accessory pigment from
Glenodinium
species of dinoflagellates, which is excited by the 488-nm light of an Argon ion laser and fluoresces at 675 nm.
Therefore, PerCP-labelled antibodies can be used with FITC- and R-PE-labelled reagents in most single-laser flow cytometers with no significant spectral overlap of PerCP fluorescence with that of FITC or R-PE.
商品描述
SAv-PerCP is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.
PerCP is a photosynthetic accessory pigment from
Glenodinium
species of dinoflagellates, which is excited by the 488-nm light of an Argon ion laser and fluoresces at 675 nm.
Therefore, PerCP-labelled antibodies can be used with FITC- and R-PE-labelled reagents in most single-laser flow cytometers with no significant spectral overlap of PerCP fluorescence with that of FITC or R-PE.
克隆号
(RUO)
浓度
0.2 mg/ml
产品详情
PerCP
PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP
Blue 488 nm
481 nm
675 nm
应用
实验应用
Flow cytometry (Routinely Tested)
目标/特异性
Streptavidin
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Afar B, Merrill J, Clark EA. Detection of lymphocyte subsets using three-color/single-laser flow cytometry and the fluorescent dye peridinin chlorophyll-alpha protein. J Clin Immunol. 1991; 11(5):254-261. (Biology).
2. Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J. Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets. Cytometry. 1996; 23(3):205-217. (Biology).
3. Shapiro HM. Practical Flow Cytometry, 3rd Edition. New York: Wiley-Liss, Inc; 1995:280-281.
4. Waggoner AS, Ernst LA, Chen CH, Rechtenwald DJ. PE-CY5. A new fluorescent antibody label for three-color flow cytometry with a single laser. Ann N Y Acad Sci. 1993; 677:185-193. (Biology).
参考图片
PerCP spectra. The absorption spectrum of Streptavidin-PerCP is presented in the left panel. The corresponding emission spectrum, at the excitation wavelength of 488 nm, appears in the right panel.
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