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BD Pharmingen- Streptavidin APC-Cy-7_BD Pharmingen

艾美捷科技 • 2025年08月21日 15:21:43 • 抗体 • 阅读 7 • 标签
产品信息
荧光素标记
APC-Cy7
抗原名称
Streptavidin
简单描述
Sav-APC-Cy7 is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. SAv-APC-Cy7/biotin conjugates can be used with APC-conjugated reagents to provide two independent staining parameters from a HeNe laser. When choosing reagents for a multicolor staining protocol, we recommend that the APC-Cy7 fluorochrome be reserved for detection of high-density antigens to assure adequate discrimination of antigen expression.
商品描述
Sav-APC-Cy7 is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. SAv-APC-Cy7/biotin conjugates can be used with APC-conjugated reagents to provide two independent staining parameters from a HeNe laser. When choosing reagents for a multicolor staining protocol, we recommend that the APC-Cy7 fluorochrome be reserved for detection of high-density antigens to assure adequate discrimination of antigen expression.
克隆号
(RUO)
浓度
0.2 mg/ml
产品详情
APC-Cy7
APC-Cy7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC-Cy7
Red 627-640 nm
651 nm
779 nm
应用
实验应用
Flow cytometry (Routinely Tested)
目标/特异性
Streptavidin
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Beavis AJ, Pennline KJ. Allo-7: a new fluorescent tandem dye for use in flow cytometry. Cytometry. 1996; 24(4):390-395. (Biology). 2. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cy7PE and Cy7APC: bright new probes for immunofluorescence. Cytometry. 1996; 24(3):191-197. (Biology).

参考图片

APC-Cy7 spectra. The absorption spectrum of SAv-APC-Cy7 is presented with the corresponding emission spectrum, at the excitation wavelength of 635 nm.

Sample staining on thymocytes (left panel) and splenocytes (right panel). BALB/c mouse thymocytes were stained with APC-conjugated anti-mouse CD4 (clone RM4-5, Cat. No. 553051) and biotinylated anti-mouse CD8a (clone 53-6.7, Cat. No. 553028/553029) (left panel). BALB/c mouse splenocytes were stained with APC-conjugated anti-mouse CD3e (clone 145-2C11, Cat. No. 553066) and biotinylated anti-mouse CD45R/B220 (clone RA3-6B2, Cat. No. 553085/553086) (right panel). The biotin conjugates were revealed with SAv-APC-Cy7. Experiements were performed on a dual-laser FACSVantage™ flow cytometry system equipped with a HeNe laser. APC-Cy7 fluorescence was collected in the FL4 PMT channel, and APC fluorescence was collected in the FL5 PMT channel.

APC-Cy7 spectra. The absorption spectrum of SAv-APC-Cy7 is presented with the corresponding emission spectrum, at the excitation wavelength of 635 nm.

Sample staining on mouse thymocytes (left panel) and splenocytes (right panel). BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051) and Biotin Rat Anti-Mouse CD8a (Cat. No. 553028/553029). BALB/c mouse splenocytes were stained with APC Hamster Anti-Mouse CD3e (Cat. No. 553066) and Biotin Rat Anti-Mouse CD45R/B220 (Cat. No. 553085/553086). The biotin conjugates were visualized with Streptavidin APC-Cy™7 (Cat. No.554063). Experiments were performed on a dual-laser FACSVantage™ flow cytometry system equipped with a HeNe laser. APC-Cy7 fluorescence was collected in the FL4 PMT channel, and APC fluorescence was collected in the FL5 PMT channel.

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