BD Pharmingen- Purified Mouse Anti-Human MLH1

BD Pharmingen- Purified Mouse Anti-Human MLH1_BD Pharmingen

艾美捷科技 • 2025年08月21日 15:29:14 • 抗体 • 阅读 8 • 标签

产品信息

抗原名称

MLH-1

宿主

Mouse IgG2a, κ

免疫原

Recombinant Human MLH

简单描述

The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. DNA repair enzymes are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex, it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen.

商品描述

G168-728 The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. DNA repair enzymes are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex, it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen.

同种型

Mouse IgG2a, κ

克隆号

克隆 G168-728 (RUO)

浓度

0.5 mg/ml

产品详情

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

应用

实验应用

Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)

反应种属

Human (QC Testing), Mouse (Tested in Development)

目标/特异性

MLH-1

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(3) 1. Baker SM, Plug AW, Prolla TA. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet. 1996; 13(3):336-342. (Clone-specific: Immunoprecipitation). 2. Prolla TA, Christie DM, Liskay RM. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene. Mol Cell Biol. 1994; 14(1):407-415. (Biology). 3. Prolla TA, Pang Q, Alani E, Kolodner RD, Liskay RM. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994; 265(5175):1091-1093. (Biology).

BD Pharmingen- Purified Mouse Anti-Human MLH1_BD Pharmingen

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