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BD Pharmingen- Purified Mouse Anti-p27 -Kip1_BD Pharmingen

艾美捷科技 • 2025年08月21日 15:27:37 • 抗体 • 阅读 7 • 标签
产品信息
抗原名称
p27Kip1 (CDKN1b)
宿主
Mouse IgG1
免疫原
Mouse p27 [Kip1] (full-length) Recombinant Protein
简单描述
Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle.  These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes. These include p15, p16, p18, p19, p21 and p27 [Kip1]. p27 [Kip1] has been shown to inhibit the activity of multiple cyclin-cdk complexes, including cyclin D-cdk4, cyclin E-cdk2 and cyclin A-cdk2. p27 [Kip1] is a 27 kD protein which shares N-terminal sequence homology with p21, and like p21, p27 [Kip1] contains a nuclear localization signal in its C-terminal region. IL-2 activation of T cells has been reported to lead to a decrease in p27 [Kip1] and entry into S phase. Removal of IL-2 from T cell cultures results in increased levels of p27 [Kip1] and cell quiescence.
商品描述
G173-524 Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle.  These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes. These include p15, p16, p18, p19, p21 and p27 [Kip1]. p27 [Kip1] has been shown to inhibit the activity of multiple cyclin-cdk complexes, including cyclin D-cdk4, cyclin E-cdk2 and cyclin A-cdk2. p27 [Kip1] is a 27 kD protein which shares N-terminal sequence homology with p21, and like p21, p27 [Kip1] contains a nuclear localization signal in its C-terminal region. IL-2 activation of T cells has been reported to lead to a decrease in p27 [Kip1] and entry into S phase. Removal of IL-2 from T cell cultures results in increased levels of p27 [Kip1] and cell quiescence.
同种型
Mouse IgG1
克隆号
克隆 G173-524 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Bioimaging, Immunoprecipitation (Tested During Development)
反应种属
Mouse (QC Testing), Human (Tested in Development)
目标/特异性
p27Kip1 (CDKN1b)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Gorospe M, Liu Y, Xu Q, Chrest FJ, Holbrook NJ. Inhibition of G1 cyclin-dependent kinase activity during growth arrest of human breast carcinoma cells by prostaglandin A2. Mol Cell Biol. 1996; 16(3):762-770. (Biology). 2. Nourse J, Firpo E, Flanagan WM, et al. Interleukin-2-mediated elimination of the p27Kip1 cyclin-dependent kinase inhibitor prevented by rapamycin. Nature. 1994; 372(6506):570-573. (Biology). 3. Polyak K, Kato JY, Solomon MJ, et al. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest. Genes Dev. 1994; 8(1):9-22. (Biology). 4. Polyak K, Lee MH, Erdjument-Bromage H, et al. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell. 1994; 78(1):59-66. (Biology). 5. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). 6. Toyoshima H, Hunter T. p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell. 1994; 78(1):67-74. (Biology).

参考图片

Immunoprecipitation/Western Blot analysis of p27 [Kip1]. Lanes 1 and 2, Equal amounts of protein (25 µg/lane) from BALB/c 3T3 cell lysates of quiescent (lane 1) and proliferating (lane 2) cells were seperated by SDS-PAGE and were probed with clone G173-524 (Cat. No. 554069). Cells may be made quiescent by techniques such as serum starvation. The antibody identifies p27 [Kip1] as a 27 kDa band and demonstrates that the level of p27 [Kip1] is higher during cell quiescence than during cell proliferation. Lane 3, lysate from quiescent BALB/c 3T3 cells was immunoprecipitated with clone G173-524. The immune complex was seperated by SDS-PAGE and p27 [Kip1] was detected by western blot analysis with clone G173-524.

Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-p27 [Kip1] antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) cells and worked with both the Triton™ X-100 and alcohol perm protocols (see Recommended Assay Procedure).

Immunoprecipitation/Western Blot analysis of p27 [Kip1]. Lanes 1 and 2, Equal amounts of protein (25 µg/lane) from BALB/c 3T3 cell lysates of quiescent (lane 1) and proliferating (lane 2) cells were seperated by SDS-PAGE and were probed with clone G173-524 (Cat. No. 554069). Cells may be made quiescent by techniques such as serum starvation.  The antibody identifies p27 [Kip1] as a 27 kDa band and demonstrates that the level of p27 [Kip1] is higher during cell quiescence than during cell proliferation. Lane 3, lysate from quiescent BALB/c 3T3 cells was immunoprecipitated with clone G173-524. The immune complex was seperated by SDS-PAGE and p27 [Kip1] was detected by western blot analysis with clone G173-524.

Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well.  After overnight incubation, cells were stained using the alcohol perm protocol and the anti-p27 [Kip1] antibody.  The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen).  The image was taken on a BD Pathway™ 855 Bioimager using a 20x objective.  This antibody also stained A549 (ATCC CCL-185) cells and worked with both the Triton™ X-100 and alcohol perm protocols (see Recommended Assay Procedure).

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