产品信息
抗原名称
MyoD
宿主
Mouse IgG1, κ
免疫原
Recombinant Mouse MyoD
简单描述
MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development; for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade.
The epitope for MoAb 5.8A has been mapped to amino acids 180-189 of mouse MyoD.
商品描述
5.8A
MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development; for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade.
The epitope for MoAb 5.8A has been mapped to amino acids 180-189 of mouse MyoD.
同种型
Mouse IgG1, κ
克隆号
克隆 5.8A (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
反应种属
Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
目标/特异性
MyoD
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Davis RL, Weintraub H, Lassar AB. Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell. 1987; 51(6):987-1000. (Biology).
2. Dias P, Dilling M, Houghton P. The molecular basis of skeletal muscle differentiation.. Semin Diagn Pathol. 1994; 11(1):3-14. (Biology).
3. Dias P, Parham DM, Shapiro DN, Tapscott SJ, Houghton PJ. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Res. 1992; 52(23):6431-6439. (Clone-specific).
4. Gredinger E, Gerber AN, Tamir Y, Tapscott SJ, Bengal E. Mitogen-activated protein kinase pathway is involved in the differentiation of muscle cells. J Biol Chem. 1998; 273(17):10436-10444. (Biology).
5. Rawls A, Valdez MR, Zhang W, Richardson J, Klein WH, Olson EN. Overlapping functions of the myogenic bHLH genes MRF4 and MyoD revealed in double mutant mice. Development. 1998; 125(13):2349-2358. (Biology).
参考图片
Western blot analysis of MyoD expression on Rh30 Rhabdomyosarcoma cells. Rh30 cell lysate was stained with Purified Mouse Anti-MyoD (Cat. No. 554130), followed by HRP Goat Anti-Mouse Ig (Cat. No. 554130) for 1 hour at room temperature. MyoD is identified as a 45-48 kDa band.
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