Rb

Mouse IgG1

Human Rb aa. 332-344

Members of the retinoblastoma (Rb) family, including the related proteins p107 and p130, share several properties, including the ability to regulate E2Fdependent transcription and to regulate cell-cycle progression. The Rb gene product is a phosphoprotein that is expressed in most normal cells of vertebrates. Rb acts as a tumor suppressor by providing a cell cycle checkpoint between the G1 and S phases. The active, underphosphorylated form of Rb (Rb or pRb) is primarily found in resting or fully differentiated cells. The activity of Rb is negatively regulated by cyclin dependent kinases, which phosphorylate Rb in late G1. Thus, the hyperphosphorylated form (ppRb) is primarily found in proliferating cells. pRB inactivation is a critical step leading to S-phase commitment at the G1 checkpoint of the cell cycle. In addition, the underphosphorylated form of Rb may bind to viral oncogenes such as SV40 large T Ag, adenoviral EIA and HPV-E7, which may contribute to the transforming activity of these viral oncoproteins. G3-245 was made using a Trp-E-Rb fusion protein as immunogen and recognizes an epitope between amino acids 332-344 (DARLFDHDKTLQ) of the human retinoblastoma protein (pp110-114 Rb).  In western blot analysis, Rb migrates as multiple closely-spaced bands between 110-116 kD on SDS-PAGE.  The bands represent different Rb phosphorylation states where the level of Rb phosphorylation can be cell cycle and/or cell-type dependent (i.e all forms may not be seen in all cell types that express Rb).  G3-245 has been reported to recognize human, monkey, mouse, rat, mink and a putative quail Rb.  This antibody has also been referred to as Mh-RB-02,20 and mAb-245.

G3-245 Members of the retinoblastoma (Rb) family, including the related proteins p107 and p130, share several properties, including the ability to regulate E2Fdependent transcription and to regulate cell-cycle progression. The Rb gene product is a phosphoprotein that is expressed in most normal cells of vertebrates. Rb acts as a tumor suppressor by providing a cell cycle checkpoint between the G1 and S phases. The active, underphosphorylated form of Rb (Rb or pRb) is primarily found in resting or fully differentiated cells. The activity of Rb is negatively regulated by cyclin dependent kinases, which phosphorylate Rb in late G1. Thus, the hyperphosphorylated form (ppRb) is primarily found in proliferating cells. pRB inactivation is a critical step leading to S-phase commitment at the G1 checkpoint of the cell cycle. In addition, the underphosphorylated form of Rb may bind to viral oncogenes such as SV40 large T Ag, adenoviral EIA and HPV-E7, which may contribute to the transforming activity of these viral oncoproteins. G3-245 was made using a Trp-E-Rb fusion protein as immunogen and recognizes an epitope between amino acids 332-344 (DARLFDHDKTLQ) of the human retinoblastoma protein (pp110-114 Rb).  In western blot analysis, Rb migrates as multiple closely-spaced bands between 110-116 kD on SDS-PAGE.  The bands represent different Rb phosphorylation states where the level of Rb phosphorylation can be cell cycle and/or cell-type dependent (i.e all forms may not be seen in all cell types that express Rb).  G3-245 has been reported to recognize human, monkey, mouse, rat, mink and a putative quail Rb.  This antibody has also been referred to as Mh-RB-02,20 and mAb-245.

Mouse IgG1

克隆 G3-245 (RUO)

0.5 mg/ml

Intracellular staining (flow cytometry), Western blot (Routinely Tested), Bioimaging (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunoprecipitation (Reported)

Human (QC Testing), Mouse, Rat, Monkey, Quail, Mink (Reported)

Rb

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(9) 1. Bignon YJ, Chen Y, Chang CY, et al. Expression of a retinoblastoma transgene results in dwarf mice. Genes Dev. 1993; 7(9):1654-1662. (Clone-specific: Western blot). 2. Cance WG, Brennan MF, Dudas ME, Huang CM, Cordon-Cardo C. Altered expression of the retinoblastoma gene product in human sarcomas. N Engl J Med. 1990; 323(21):1457-1462. (Clone-specific: Immunohistochemistry). 3. DeCaprio JA, Ludlow JW, Figge J, et al. SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene. Cell. 1988; 54(2):275-283. (Clone-specific: Immunoprecipitation, Western blot). 4. Dowdy SF, Hinds PW, Louie K, Reed SI, Arnold A, Weinberg RA. Physical interaction of the retinoblastoma protein with human D cyclins. Cell. 1993; 73(3):499-511. (Clone-specific: Flow cytometry). 5. Huang S, Lee WH, Lee EY. A cellular protein that competes with SV40 T antigen for binding to the retinoblastoma gene product. Nature. 1991; 350(6314):160-162. (Clone-specific: Western blot). 6. Mittnacht S, Weinberg RA. G1/S phosphorylation of the retinoblastoma protein is associated with an altered affinity for the nuclear compartment. Cell. 1991; 65(3):381-393. (Clone-specific: Fluorescence microscopy). 7. Niwa S, Ueno S, Shirasu R. Alteration of pRb expression in the development of rat tongue carcinoma induced by 4-nitroquinoline 1-oxide. Oral Oncol. 2001; 37(7):579-585. (Clone-specific: Immunofluorescence). 8. Nork TM, Millecchia LL, Poulsen G. Immunolocalization of the retinoblastoma protein in the human eye and in retinoblastoma. Invest Ophthalmol Vis Sci. 1994; 35(6):2682-2692. (Clone-specific: Immunohistochemistry). 9. Riley DJ, Lee EY, Lee WH. The retinoblastoma protein: more than a tumor suppressor. Annu Rev Cell Biol. 1994; 10:1-29. (Biology).