p53

Mouse IgG2b

SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4)

The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers.1 Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and it forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. p53 migrates at a reduced molecular weight of 53 kDa. PAb 122 recognizes mammalian p53, including mouse, rat, hamster, monkey and human p53.2 Cells from the SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4) were used as immunogen. PAb 122 was originally evaluated by ELISA, immunoprecipitation, radioimmunoassays and indirect immunofluorescence of cultured cells.

PAb 122 The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers.1 Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and it forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. p53 migrates at a reduced molecular weight of 53 kDa. PAb 122 recognizes mammalian p53, including mouse, rat, hamster, monkey and human p53.2 Cells from the SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4) were used as immunogen. PAb 122 was originally evaluated by ELISA, immunoprecipitation, radioimmunoassays and indirect immunofluorescence of cultured cells.

Mouse IgG2b

克隆 PAb 122 (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Flow cytometry, Immunofluorescence, Immunoprecipitation (Tested During Development)

Monkey (QC Testing), Human, Mouse, Rat, Hamster (Reported)

p53

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Diller L, Kassel J, Nelson CE, et al. p53 functions as a cell cycle control protein in osteosarcomas. Mol Cell Biol. 1990; 10(11):5772-5781. (Clone-specific: Flow cytometry, Immunofluorescence). 2. Gurney EG, Harrison RO, Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980; 34(3):752-763. (Clone-specific: Immunofluorescence, Immunoprecipitation). 3. Legros Y, Lacabanne V, d'Agay MF, Larsen CJ, Pla M, Soussi T. Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells. Bull Cancer. 1993; 80(2):102-110. (Clone-specific: Western blot). 4. Vogelstein B. Cancer. A deadly inheritance. Nature. 1990; 348(6303):681-682. (Biology). 5. Yewdell JW, Gannon JV, Lane DP. Monoclonal antibody analysis of p53 expression in normal and transformed cells. J Virol. 1986; 59(2):444-452. (Clone-specific: Immunofluorescence, Western blot).