SV40 Large T Antigen

Mouse IgG2a

SV40-transformed BALB/c mouse cell lines

Simian virus 40 is a small DNA virus encoded by 5.2 kb of double-stranded DNA. SV40 large T antigen (T-ag) is a multifunctional ~85 kD phosphoprotein, which is the sole viral protein required for SV40 replication. All other factors are provided by the infected host cell. In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines. Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated. The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines. SV40 T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter. In addition to transcriptional regulation, T-ag is involved in viral DNA replication. Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, and ATPase and helicase activities. Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis and provision of a host-range function for viral replication. However, all functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation and adenylation. T-ag exists in monomeric as well as polymeric forms and associates with the tumor suppressor proteins p53 and Rb (retinoblastoma protein). Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface. PAb 101 recognizes a C-terminal epitope within the last 190 amino acids of T-ag. PAb 101 was originally known as Clone 7. Studies have suggested that PAb 101 binds the strongest to mature T-ag. PAb 101 (i.e., Clone 7) was developed along with a panel of monoclonal antibodies where SV40-transformed BALB/c mouse cell lines (SVT2 or B4) were used as immunogens. The specificity of the antibody was originally characterized by a variety of techniques using SV40-infected and SV40-transformed cells.

PAb 101 Simian virus 40 is a small DNA virus encoded by 5.2 kb of double-stranded DNA. SV40 large T antigen (T-ag) is a multifunctional ~85 kD phosphoprotein, which is the sole viral protein required for SV40 replication. All other factors are provided by the infected host cell. In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines. Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated. The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines. SV40 T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter. In addition to transcriptional regulation, T-ag is involved in viral DNA replication. Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, and ATPase and helicase activities. Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis and provision of a host-range function for viral replication. However, all functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation and adenylation. T-ag exists in monomeric as well as polymeric forms and associates with the tumor suppressor proteins p53 and Rb (retinoblastoma protein). Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface. PAb 101 recognizes a C-terminal epitope within the last 190 amino acids of T-ag. PAb 101 was originally known as Clone 7. Studies have suggested that PAb 101 binds the strongest to mature T-ag. PAb 101 (i.e., Clone 7) was developed along with a panel of monoclonal antibodies where SV40-transformed BALB/c mouse cell lines (SVT2 or B4) were used as immunogens. The specificity of the antibody was originally characterized by a variety of techniques using SV40-infected and SV40-transformed cells.

Mouse IgG2a

克隆 PAb 101 (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Reported)

Viral (QC Testing)

SV40 Large T Antigen

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(9) 1. Carroll RB, Gurney EG. Time-dependent maturation of the simian virus 40 large T antigen-p53 complex studied by using monoclonal antibodies. J Virol. 1982; 44(2):565-573. (Clone-specific). 2. Gurney EG, Harrison RO, Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980; 34(3):752-763. (Immunogen: Immunofluorescence, Immunoprecipitation). 3. Gurney EG, Tamowski S, Deppert W. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J Virol. 1986; 57(3):1168-1172. (Clone-specific: Immunoprecipitation, Western blot). 4. Li M, Hu J, Heermeier K, Hennighausen L, Furth PA. Expression of a viral oncoprotein during mammary gland development alters cell fate and function: induction of p53-independent apoptosis is followed by impaired milk protein production in surviving cells. Cell Growth Differ. 1996; 270(1):3-11. (Clone-specific: Immunohistochemistry). 5. Prives C, Covey L, Scheller A, Gluzman Y. DNA-binding properties of simian virus 40 T-antigen mutants defective in viral DNA replication. Mol Cell Biol. 1983; 3(11):1958-1966. (Clone-specific). 6. Scheller A, Covey L, Barnet B, Prives C. A small subclass of SV40 T antigen binds to the viral origin of replication. Cell. 1982; 29(2):375-383. (Clone-specific). 7. Stahl H, Dröge P, Knippers R. DNA helicase activity of SV40 large tumor antigen. EMBO J. 1986; 5(8):1939-1944. (Clone-specific). 8. Tack LC, Wright JH, Gurney EG. Alterations in the structure of new and old forms of simian virus 40 large T antigen (T) defined by age-dependent epitope changes: new T is the same as ATPase-active T. J Virol. 1989; 63(5):2352-2358. (Clone-specific: Immunoprecipitation). 9. Tack LC, Wright JH, Gurney EG. Characterization of simian virus 40 large T antigen by using different monoclonal antibodies: T-p53 complexes are preferentially ATPase active and adenylylated. J Virol. 1988; 62(3):1028-1037. (Clone-specific: Immunoprecipitation).