1. 首页
  2. 抗体
  3. 正文

BD Pharmingen- Purified Mouse Anti-Human Underphosphorylated Retinoblastoma Protein -RB_BD Pharmingen

艾美捷科技 • 2025年08月21日 15:45:25 • 抗体 • 阅读 16 • 标签
产品信息
抗原名称
Rb
宿主
Mouse IgG1
免疫原
Recombinant full-length human Rb
简单描述
The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as a tumor suppressor gene product. An underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the hyperphosphorylated form is present in proliferating cells. Only the underphosphorylated form of Rb binds specifically to viral oncogenes such as SV40 large T, adenoviral EIA and HPV-E7. This interaction may partially contribute to the transforming activity of these viral oncoproteins. Rb also interacts with several cyclins including A, D, and E as well as the transcriptional activator E2F. The importance of these interactions for the biological function of Rb is still being elucidated. G99-549 recognizes only the fast migrating, underphosphorylated form of Rb protein. G99-549 cross-reacts with mouse underphosphorylated Rb. The epitope has been mapped to amino acids 514-610 (box A) of human Rb. Recombinant full-length human Rb was produced in the baculovirus expression vector system (BEVS), purified and used as immunogen.
商品描述
G99-549 The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as a tumor suppressor gene product. An underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the hyperphosphorylated form is present in proliferating cells. Only the underphosphorylated form of Rb binds specifically to viral oncogenes such as SV40 large T, adenoviral EIA and HPV-E7. This interaction may partially contribute to the transforming activity of these viral oncoproteins. Rb also interacts with several cyclins including A, D, and E as well as the transcriptional activator E2F. The importance of these interactions for the biological function of Rb is still being elucidated. G99-549 recognizes only the fast migrating, underphosphorylated form of Rb protein. G99-549 cross-reacts with mouse underphosphorylated Rb. The epitope has been mapped to amino acids 514-610 (box A) of human Rb. Recombinant full-length human Rb was produced in the baculovirus expression vector system (BEVS), purified and used as immunogen.
同种型
Mouse IgG1
克隆号
克隆 G99-549 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Immunoprecipitation (Routinely Tested)
反应种属
Human (QC Testing), Mouse (Tested in Development)
目标/特异性
Rb
背景
别名
RB (a.a. 514-610)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Cordon-Cardo C, Richon VM. Expression of the retinoblastoma protein is regulated in normal human tissues. Am J Pathol. 1994; 144(3):500-510. (Biology). 2. Dunaief JL, Strober BE, Guha S, et al. The retinoblastoma protein and BRG1 form a complex and cooperate to induce cell cycle arrest. Cell. 1994; 79(1):119-130. (Clone-specific: Western blot). 3. Hollingsworth RE Jr, Chen PL, Lee WH. Integration of cell cycle control with transcriptional regulation by the retinoblastoma protein. Curr Opin Cell Biol. 1993; 5(2):194-200. (Biology). 4. Livingston DM. Functional analysis of the retinoblastoma gene product and of RB-SV40 T antigen complexes. Cancer Surv. 1992; 12:153-160. (Biology). 5. Riley DJ, Lee EY, Lee WH. The retinoblastoma protein: more than a tumor suppressor. Annu Rev Cell Biol. 1994; 10:1-29. (Biology). 6. Weinberg RA. Tumor suppressor genes. Science. 1991; 254(5035):1138-1146. (Biology).

参考图片

Selective binding of G99-549 to underphosphorylated Rb. MOLT-4 cells (ATCC CRL-1582, Human T lymphoblast) were labeled with [32P]-orthophosphate and immunoprecipitated with either clone G99-549 (lanes 1 & 3) or clone G3-245 (lanes 2 & 4). G99-549 (Cat. No. 554164) recognizes only underphosphorylated Rb whereas G3-245 (Cat. No. 554136) recognizes all species of Rb (highly phosphorylated, phosphorylated and under phosphorylated). Lanes 1 & 2 - When P32-labeled IP extracts are run on a gel and detected via autoradiography, the radioactive label is greatly reduced in the G99-549 (lane 1) compared to the G3-245 (lane 2) immunoprecipitates. Lanes 3 & 4 - A western blot of the same gel probing for total Rb (Clone G3-245) shows that underphosphorylated Rb is present in the G99-549 immunoprecipitates (lane 3) whereas underphosphorylated, phosphorylated, and highly phosphorylated forms are present in the G3-245 immunoprecipitates (lane 4). pRb, underphosphorylated Rb. ppRb, phosphorylated and highly phosphorylated forms of Rb.

文档下载: W 导出为BD Pharmingen- Purified Mouse Anti-Human Underphosphorylated Retinoblastoma Protein -RB_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9530.html

赞 (16)
打赏 微信扫一扫
« 上一篇 2025年08月21日 15:43:50
下一篇 » 2025年08月21日 15:47:26

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();