BD Pharmingen- Purified Mouse Anti-L1 Protein of Human Papilloma Virus_BD Pharmingen
产品信息
抗原名称
HPV-16 L1
宿主
Mouse IgG2a, κ
简单描述
More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.
商品描述
CAMVIR-1
More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.
同种型
Mouse IgG2a, κ
克隆号
克隆 CAMVIR-1 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Immunohistochemistry-formalin (antigen retrieval required) (Routinely Tested), Immunofluorescence, Western blot (Reported)
反应种属
Human (QC Testing)
目标/特异性
HPV-16 L1
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Doorbar J, Ely S, Coleman N, Hibma M, Davies DH, Crawford L. Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein. Virology. 1992; 187(1):353-359. (Biology).
2. McLean CS, Churcher MJ, Meinke J. Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. J Clin Pathol. 1990; 43(6):488-492. (Immunogen: Immunofluorescence, Western blot).
3. Zhou J, Sun XY, Stenzel DJ, Frazer IH. Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles. Virology. 1991; 185(1):251-257. (Biology).
4. de Villiers EM. Heterogeneity of the human papillomavirus group. J Virol. 1989; 63(11):4898-4903. (Biology).
参考图片
Immunohistochemical staining for human papilloma virus (HPV) type 16, L1 protein. A formalin-fixed, paraffin-embedded tissue section from human cervix infrected with HPV type 16 was stained with either the Purified Mouse Anti-HPV type 16 L1 antibody (Left panel ) or with a mouse IgG2a isotype control (Right panel).
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