Cyclin B1

Mouse IgG1

Human Cyclin B1 Recombinant Protein

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catlytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins, such as p21 and p27 [Kip1].  Cyclin B1 is a mitotic cyclin, where expression is normally low in G0/G1, increases in S and is maximal during the G2/M phase. Cyclin B1 is rapidly degraded at the end of mitosis, and is required for cells to exit from mitosis.  This antibody has been reported to react to hamster and mouse cyclin B1.  In addition, the GNS-1 antibody has been reported to recognize an epitope between amino acids 1-21 of human cyclin B1.

GNS-1 Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catlytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins, such as p21 and p27 [Kip1].  Cyclin B1 is a mitotic cyclin, where expression is normally low in G0/G1, increases in S and is maximal during the G2/M phase. Cyclin B1 is rapidly degraded at the end of mitosis, and is required for cells to exit from mitosis.  This antibody has been reported to react to hamster and mouse cyclin B1.  In addition, the GNS-1 antibody has been reported to recognize an epitope between amino acids 1-21 of human cyclin B1.

Mouse IgG1

克隆 GNS-1 (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Bioimaging (Tested During Development), Flow cytometry, Fluorescence microscopy, Immunohistochemistry, Immunoprecipitation (Reported)

Human (QC Testing), Mouse,Hamster (Reported)

Cyclin B1

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(13) 1. Cao L, Faha B, Dembski M, Tsai LH, Harlow E, Dyson N. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. Nature. 1992; 355(6356):176-179. (Clone-specific). 2. Coleman TR, Tang Z, Dunphy WG. Negative regulation of the wee1 protein kinase by direct action of the nim1/cdr1 mitotic inducer. Cell. 1993; 72(6):919-929. (Clone-specific: Western blot). 3. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry. 1996; 25(1):1-13. (Methodology: Flow cytometry). 4. Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science. 1992; 255(5040):87-90. (Biology). 5. Faha B, Harlow E, Lees E. The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2. J Virol. 1993; 67(5):2456-2465. (Clone-specific: Immunoprecipitation). 6. Gong J, Ardelt B, Traganos F, Darzynkiewicz Z. Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines. Cancer Res. 1994; 54(16):4285-4288. (Clone-specific: Flow cytometry). 7. Gong J, Traganos F, Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. Exp Cell Res. 1995; 220(1):226-231. (Clone-specific: Flow cytometry, Western blot). 8. Gong J, Traganos F, Darzynkiewicz Z. Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements. Cancer Res. 1993; 53(21):5096-5099. (Clone-specific: Flow cytometry). 9. Gong J, Traganos F, and Darzynkiewicz Z. Expression of cyclins B and E in individual MOLT-4 cells and in stimulated human lymphocytes during progression through the cell cycle. Int J Oncol. 1993; 3:1037-1042. (Clone-specific: Flow cytometry). 10. Kung AL, Sherwood SW, Schimke RT. Differences in the regulation of protein synthesis, cyclin B accumulation, and cellular growth in response to the inhibition of DNA synthesis in Chinese hamster ovary and HeLa S3 cells. J Biol Chem. 1993; 268(31):23072-23080. (Clone-specific: Flow cytometry). 11. Sherwood SW, Kung AL, Roitelman J, Simoni RD, Schimke RT. In vivo inhibition of cyclin B degradation and induction of cell-cycle arrest in mammalian cells by the neutral cysteine protease inhibitor N-acetylleucylleucylnorleucinal. Proc Natl Acad Sci U S A. 1993; 90(8):3353-3357. (Clone-specific: Fluorescence microscopy, Immunohistochemistry, Western blot). 12. Sherwood SW, Rush DF, Kung AL, Schimke RT. Cyclin B1 expression in HeLa S3 cells studied by flow cytometry. Exp Cell Res. 1994; 211(2):275-281. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunohistochemistry). 13. Zhang H, Xiong Y, Beach D. Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol Biol Cell. 1993; 4(9):897-906. (Clone-specific: Flow cytometry, Immunoprecipitation).