DCC

Mouse IgG1

Recombinant Human DCC

One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas. This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kDa  glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family.  DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas.  The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin. Clone G97-449 recognizes human DCC.  A truncated recombinant protein containing the intracellular domain of the human DCC was used as immunogen.

G97-449 One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas. This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kDa  glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family.  DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas.  The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin. Clone G97-449 recognizes human DCC.  A truncated recombinant protein containing the intracellular domain of the human DCC was used as immunogen.

Mouse IgG1

克隆 G97-449 (RUO)

0.5 mg/ml

Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Reported)

Human (QC Testing)

DCC

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Fearon ER, Cho KR, Nigro JM. Identification of a chromosome 18q gene that is altered in colorectal cancers. Science. 1990; 247(4938):49-56. (Biology). 2. Fearon ER, Hamilton SR, Vogelstein B. Clonal analysis of human colorectal tumors. Science. 1987; 238(4824):193-197. (Biology). 3. Reale MA, Hu G, Zafar AI, Getzenberg RH, Levine SM, Fearon ER. Expression and alternative splicing of the deleted in colorectal cancer (DCC) gene in normal and malignant tissues. Cancer Res. 1994; 54(16):4493-4501. (Biology: Immunoprecipitation, Western blot). 4. Shibata D, Reale MA, Lavin P. The DCC protein and prognosis in colorectal cancer. N Engl J Med. 1996; 335(23):1727-1732. (Clone-specific: Immunohistochemistry). 5. Vogelstein B, Fearon ER, Hamilton SR . Genetic alterations during colorectal-tumor development. N Engl J Med. 1988; 319(9):525-532. (Biology).