Androgen Receptor

Mouse IgG1

Purified human androgen receptor recombinant protein

The androgen receptor (AR) mediating androgen effects is a member of the steroid and thyroid hormone receptor gene superfamily encoding ligand-dependent nuclear transcription factors. They can be divided into three main domains, an N-terminal domain which modulates transcription efficiency, a central DNA domain which binds to a target gene hormone response element, and a C-terminal hormone binding domain. Androgens are necessary for normal male development and function. They exert their effects on target tissue through binding to the AR, followed by association of the AR complex with specific binding sites on DNA. This AR complex may induce or repress gene transcription. Mutations in the AR gene are associated with androgen insensitivity syndrome, a disorder that causes XY genotypic males to develop as phenotypic females because of their inability to respond to androgens. AR mutations have also been identified in human prostate cancer specimens and the LNCaP human prostate cancer cell line. Monoclonal and polyclonal antibodies to the AR have identified AR-positive cells in a variety of tissues including male and female sexual organs, kidney, liver, adrenal cortex, pituitary gland, skeletal, cardiac, and smooth muscle cells. The androgen receptor migrates at a molecular weight of ~100 kD in SDS-PAGE. G122–25 recognizes an epitope localized within the DNA-binding domain (between amino acids 486 and 651) of the human androgen receptor. Additionally, G122–25 has been shown to recognize androgen receptors in frozen rat prostate tissue sections. G122–25 does not cross-react with estrogen or progesterone receptors. Purified recombinant human androgen receptor protein was used as immunogen.

G122-25 The androgen receptor (AR) mediating androgen effects is a member of the steroid and thyroid hormone receptor gene superfamily encoding ligand-dependent nuclear transcription factors. They can be divided into three main domains, an N-terminal domain which modulates transcription efficiency, a central DNA domain which binds to a target gene hormone response element, and a C-terminal hormone binding domain. Androgens are necessary for normal male development and function. They exert their effects on target tissue through binding to the AR, followed by association of the AR complex with specific binding sites on DNA. This AR complex may induce or repress gene transcription. Mutations in the AR gene are associated with androgen insensitivity syndrome, a disorder that causes XY genotypic males to develop as phenotypic females because of their inability to respond to androgens. AR mutations have also been identified in human prostate cancer specimens and the LNCaP human prostate cancer cell line. Monoclonal and polyclonal antibodies to the AR have identified AR-positive cells in a variety of tissues including male and female sexual organs, kidney, liver, adrenal cortex, pituitary gland, skeletal, cardiac, and smooth muscle cells. The androgen receptor migrates at a molecular weight of ~100 kD in SDS-PAGE. G122–25 recognizes an epitope localized within the DNA-binding domain (between amino acids 486 and 651) of the human androgen receptor. Additionally, G122–25 has been shown to recognize androgen receptors in frozen rat prostate tissue sections. G122–25 does not cross-react with estrogen or progesterone receptors. Purified recombinant human androgen receptor protein was used as immunogen.

Mouse IgG1

克隆 G122-25 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunoprecipitation (Tested During Development)

Human (QC Testing), Rat (Tested in Development)

Androgen Receptor

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(5) 1. Chang C, Wang C, DeLuca HF, Ross TK, Shih CC. Characterization of human androgen receptor overexpressed in the baculovirus system. Proc Natl Acad Sci U S A. 1992; 89(13):5946-5950. (Immunogen). 2. Fuller PJ. The steroid receptor superfamily: mechanisms of diversity. FASEB J. 1991; 5(15):3092-3099. (Biology). 3. Newmark JR, Hardy DO, Tonb DC, et al. Androgen receptor gene mutations in human prostate cancer. Proc Natl Acad Sci U S A. 1992; 89(14):6319-6323. (Biology). 4. Shih CC, Young WJ, Wang CH, et al. Monoclonal anti-androgen receptor antibodies: production, characterization and potential diagnostic applications. Mol Cell Biochem. 1999; 201(1-2):131-140. (Clone-specific: Flow cytometry). 5. Takeda H, Chodak G, Mutchnik S, Nakamoto T, Chang C. Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor. J Endocrinol. 1990; 126(1):17-25. (Biology).