BD Pharmingen- Purified Mouse Anti-Human p21_BD Pharmingen
产品信息
抗原名称
p21
宿主
Mouse IgG1
免疫原
Human p21
简单描述
p21 belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21 and p53-regulated inhibitor of Cdks (Pic1). p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. When introduced into proliferating foreskin fibroblasts, p21 inhibits DNA synthesis and cell cycle arrest. p53 can activate p21 gene expression via a p53 binding site identified in the promoter of the p21 gene.
商品描述
6B6
p21 belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21 and p53-regulated inhibitor of Cdks (Pic1). p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. When introduced into proliferating foreskin fibroblasts, p21 inhibits DNA synthesis and cell cycle arrest. p53 can activate p21 gene expression via a p53 binding site identified in the promoter of the p21 gene.
同种型
Mouse IgG1
克隆号
克隆 6B6 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Western blot (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunoprecipitation (Tested During Development)
反应种属
Human (QC Testing)
目标/特异性
p21
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(8)
1. Clarke AS, Lotz MM, Chao C, Mercurio AM. Activation of the p21 pathway of growth arrest and apoptosis by the beta 4 integrin cytoplasmic domain. J Biol Chem. 1995; 270(39):22673-22676. (Clone-specific: Immunohistochemistry).
2. Halevy O, Novitch BG, Spicer DB, et al. Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD. Science. 1995; 267(5200):1018-1021. (Clone-specific: Immunohistochemistry, Immunoprecipitation).
3. Hunter T. Braking the cycle. Cell. 1993; 75(5):839-841. (Biology).
4. Noda A, Ning Y, Venable SF, Pereira-Smith OM, Smith JR. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp Cell Res. 1994; 211(1):90-98. (Biology).
5. Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R, Beach D. p21 is a universal inhibitor of cyclin kinases. Nature. 1993; 366(6456):701-704. (Biology).
6. Zhang W, Grasso L, McClain CD. p53-independent induction of WAF1/CIP1 in human leukemia cells is correlated with growth arrest accompanying monocyte/macrophage differentiation. Cancer Res. 1995; 55(3):668-674. (Clone-specific: Western blot).
7. el-Deiry WS, Harper JW, O'Connor PM, et al. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994; 54(5):1169-1174. (Biology).
8. el-Deiry WS, Tokino T, Velculescu VE, et al. WAF1, a potential mediator of p53 tumor suppression. Cell. 1993; 75(4):817-825. (Biology).
参考图片
Western blot analysis of p21. Lysate from MCF7 breast carcinoma cells was probed with anti-human p21 (Cat. No. 15091A).
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